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Last updated: 2017/12/10
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CytoQuest™ CR - Circulating Rare Cell Positive Enrichment & Retrieval System

Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs). Three major subtypes of CRCs in translational research and clinical studies are circulating tumor cells (CTCs), circulating progenitor cells (CPCs), and circulating fetal cells (CFCs). A challenge for market adoption of CRCs is the efficient and reproducible identification, single cell isolation, and retrieval of highly pure and viable CRCs, with their applications supported by a wide repertoire of standardized, GMP grade bioreagents.

CytoQuest™ CR technology utilizes SCx™ spiral chamber, HBx™ micromixer, antibody immobilized nanoarray, and TCx™ thermal control to enable a multitude of CRC functions. SCx™ spiral chamber is equipped with a non-sticky coil and a self-contained micro-vibrator for unimpeded delivery of pretreated blood sample into the nanoarray. HBx™ micromixer provides a herringbone conduit for cell mixing. CytoChipNano is a streptavidin nanoarray which captures the CRCs for cell enumeration and single cell isolation by laser microdissection or micromanipulation. CytoChipNano CR is a specialized streptavidin nanoarray with thermo-sensitive coating which captures and releases the CRCs via a TCx™ thermal control of alternating temperatures.

Monoclonal antibodies targeting specific surface biomarkers of circulating rare cells can be flexibly interchanged to accommodate the biomarker switch in CRC progression. This results in highly pure and viable CRCs for downstream protein characterizations, gene analyses, and cell assays. Cell enumeration is currently the only FDA approved application as a prognostic marker in breast, prostate, and colorectal cancers. In contrast, effective single cell isolation and retrieval of CRCs opens up new scope of applications in the diagnostic and pharmaceutical industry.

Catalog # : M0014-04
Price: Please Inquire
Regulatory Status : CE-IVD
Subtypes of CRCs

  • Circulating Tumor Cells (CTCs)
  • Circulating Progenitor Cells (CPCs)
  • Circulating Fetal Cells (CFCs)
Applications of CTCs

Protein Characterizations
  • Immunofluorescence
  • Proximity ligation assay (PLA)
Gene Analyses
  • Fluorescent In Situ Hybridization (FISH)
  • Polymerase Chain Reaction (PCR)
      Gene Amplifications (GAs)
      Somatic Mutations (SMs)
      Single Nucleotide Polymorphisms (SNPs)
  • Comparative Genomic Hybridization (CGH)
      Copy Number Variants (CNVs)
  • Next Generation Sequencing (NGS)
Cell Assays
  • Primary cell line construction
  • Compound screening
Advantages of CytoQuest™ CR Platform

Integrated system
  • Bottle reservoir
  • Fluidic handler
  • SCx™ spiral chamber
  • TBx™ tension balancer
  • Coupler
  • HBx™ micromixer
  • Chip receptor
  • TCx™ thermal chuck
  • Touch user interface
Antibody-based, cell capture
  • Cell enumeration (CytoChipNano)
  • Single cell isolation (CytoChipNano)
  • Thermal cell release (CytoChipNano CR)

CytoQuest™ CR                     

Buffer Reservoir
Bottle Reservoir
Fluidic Handler
SCx™ Spiral
Spiral Chamber Holder
Spiral Chamber Holder
TBx™ Tension Balancer
TBx™ Tension Balancer
Chip Receptor
Chip Receptor
HBx™ Micromixer
CytoChipNano CR
TCx™ Thermal Chuck
TCx™ Thermal
Chip Opener
Chip Opener
Temperature Display
QR Code Scanner
QR Code Scanner
Power On LED
Power On LED
Touch User Interface
Touch User Interface
Hardware General Specifications

Dimensions: 565.06mm(h) x 340.8mm(d) x 271.8mm(w)
Weight: 18 kg
Power: Fluidic Handler: 100-240VAC, 50/60Hz,1.6A
Thermal Controller: 100-240VAC, 50/60Hz, 4.0A
Replace Fuse: Fluidic Handler: 1.6A(Peak)/250V
Thermal Controller: 4A (Peak)/250V
Operating Temperature: 60°F to 104°F (15°C to 40°C)
Programable Thermo Control Profile: 4-40°C, Variable Time Span
Hardware Environmental Specifications

Storage Temperature: -5°F to 158°F (-20°C to 70°C)
Operation Altitude: Up to 2000m
Operation Environment: For indoor use
Operating Humidity: 20% to 90% Relative Humidity, Non-Condensing
Storage Humidity: 20% to 95% Relative Humidity, Non-Condensing
Software Specifications

Scanner Function: Scanning Capability to Read QR Code/Barcode
Status Display: Progress and Fluid Handling
System Clean: Prime and Clean
Report Generation: Print and Export File
Touchscreen Laptop Specifications

Operating System: Windows 7/8/10 (32-bit and 64-bit)
Processor: Pentium III/Celeron 866 MHz or Equivalent
Memory: 1GB Minimum (2GB Recommended)
Free Disk Space: 4GB
Interfaces/USB Ports: USB 2.0 Port*1

Cell Enumeration
  • CytoChipNano Combo Pack (Catalog #: KA4927)
  • CytoChipNano (Catalog #: U0095)
  • SCx™ Spiral Chamber (Catalog #: U0314)
  • Plugs (Catalog #: KA4441)
Single Cell Isolation
  • CytoChipNano Combo Pack (Catalog #: KA4927)
  • CytoChipNano (Catalog #: U0095)
  • SCx™ Spiral Chamber (Catalog #: U0314)
  • Plugs (Catalog #: KA4441)
Capture & Release
  • CytoChipNano CR Combo Pack (Catalog #: KA4862)
  • CytoChipNano CR (Catalog #: U0096)
  • SCx™ Spiral Chamber (Catalog #: U0314)
  • Plugs (Catalog #: KA4441)

Circulating Epithelial Cells
  • CytoQuest™ EpCAM PanCK CD45 Antibody Kit (Catalog #: KA4439)
  • CytoQuest™ EpCAM PanCK CD45 Antibody Release Kit (Catalog #: KA4863)
  • CytoQuest™ EpCAM PanCK CD45 Mouse Antibody Kit (Catalog #: KA4576)
  • CytoQuest™ EpCAM PanCK CD45 PLS3 EMT Antibody Kit (Catalog #: KA4586)
  • CytoQuest™ EpCAM PanCK CD45 CSV EMT Antibody Kit (Catalog #: KA4817)
  • CytoQuest™ EpCAM PanCK CD45 CD44v9 EMT Antibody Kit (Catalog #: KA4897)
  • CytoQuest™ Epithelial Cell Solution (Catalog #: KA3967)
Circulating Tumor Cells
  • CytoQuest™ Choriocarcinoma HLA-G PanCK CD45 Antibody Kit (Catalog #: KA4530)
  • CytoQuest™ Colorectal Cancer EpCAM PanCK CD45 CDX2 Antibody Kit (Catalog #: KA4785)
  • CytoQuest™ Glioblastoma Multiforme CSV CSV CD45 EGFRvIII Antibody Kit (Catalog #: KA4906)
  • CytoQuest™ Glioblastoma Multiforme CSV CSV CD45 GFAP Antibody Kit (Catalog #: KA4905)
  • CytoQuest™ Liver Cancer ASGPR PanCK CD45 Antibody Kit (Catalog #: KA4573)
  • CytoQuest™ Lung Cancer EpCAM PanCK CD45 TTF1 Antibody Kit (Catalog #: KA4896)
  • CytoQuest™ Neuroblastoma CSV CSV CD45 Antibody Kit (Catalog #: KA4911)
  • CytoQuest™ Pancreatic Cancer EpCAM PanCK CD45 PDX1 Antibody Kit (Catalog #: KA4575)
  • CytoQuest™ Prostate Cancer EpCAM PanCK CD45 AR-V7 Antibody Kit (Catalog #: KA4910)
  • CytoQuest™ Prostate Cancer PSMA PanCK CD45 Antibody Kit (Catalog #: KA4574)
  • CytoQuest™ Sarcoma CSV CSV CD45 Antibody Kit (Catalog #: KA4864)
  • CytoQuest™ Universal Circulating Tumor Cell CSV CSV CD45 Antibody Kit (Catalog #: KA4818)
Circulating Cancer Stem Cells
  • CytoQuest™ Glioblastoma Multiforme CSV CD45 DBA Antibody Kit (Catalog #: KA4908)
  • CytoQuest™ Lung Cancer CSV CSV CD45 TTF1 Antibody Kit (Catalog #: KA4907)
  • CytoQuest™ Prostate Cancer CSV CSV CD45 PD-L1 Antibody Kit (Catalog #: KA4909)
  • CytoQuest™ Renal Cancer B7-H3(CD276) CD105 CD45 Antibody Kit (Catalog #: KA4577)
  • CytoQuest™ Universal Cancer Stem Cell CSV CSV CD45 Antibody Kit (Catalog #: KA4819)
Circulating Trophoblasts
  • CytoQuest™ Extravillous Trophoblast HLA-G TBA Antibody Kit (Catalog #: KA4515)
Blockers, Enhancers & Pickers
  • CytoQuest™ FcR Blocking Reagent (Catalog #: U0318)
  • CytoQuest™ MicroBlocker (Catalog #: U0335)
  • CytoQuest™ Staining Blocker (Catalog #: U0330)
  • CytoQuest™ SuperBlocker (Catalog #: U0317)
  • CytoQuest™ Single Cell Picking Kit (Catalog #: KA4578)
Mutation, Deletion, Gene Amplification, and Translocation mutaFISH™ Probes and Accessory Kits
  • mutaFISH™ AR-V7 ARwt RNA FISH Probes (Catalog #: FP0004)
  • mutaFISH™ CEP1wt CEP7wt CEP8wt CEP17wt DNA Probes (Catalog #: FP0009)
  • mutaFISH™ CEP1wt DNA Probes (Catalog #: FP0008)
  • mutaFISH™ CEP7wt DNA Probes (Catalog #: FP0006)
  • mutaFISH™ CEP8wt DNA Probes (Catalog #: FP0005)
  • mutaFISH™ CEP17wt DNA Probes (Catalog #: FP0007)
  • mutaFISH™ EGFR T790M T790wt RNA Probes (Catalog #: FP0001)
  • mutaFISH™ EGFR L858R L858wt Ex19wt RNA Probes (Catalog #: FP0002)
  • mutaFISH™ MYCNwt DNA Probes (Catalog #: FP0003)
  • mutaFISH™ DNA Accessory Kit 1 for Cell (Catalog #: KA4916)
  • mutaFISH™ DNA Accessory Kit 2 for Cell (Catalog #: KA4928)
  • mutaFISH™ RNA Accessory Kit (Catalog #: KA4915)
Gene Amplification, Translocation, and Split FISH Probes
  • ACTN4 DNA Probe (Catalog #: DF0001)
  • AR/CENXp FISH Probe (Catalog #: FG0163)
  • BRCA1/CEN17q FISH Probe (Catalog #: FG0069)
  • CD274 (PD-L1)/CEN9q FISH Probe (Catalog #: FG0160)
  • CEP8 (R6G) FISH Probe (Catalog #: FC0197)
  • EGFR/CEN7p FISH Probe (Catalog #: FG0114)
  • EML4/ALK Translocation FISH Probe (Catalog #: FT0009)
  • ERBB2 (HER2)/CEN17q FISH Probe (Catalog #: FG0166)
  • ERG Split FISH Probe (Catalog #: FS0008)
  • MYC/CEN8q FISH Probe (Catalog #: FG0117)
  • p53/CEN17q FISH Probe (Catalog #: FG0011)
  • PLS3/CENXp FISH Probe (Catalog #: FG0164)
  • PRDM14 FISH Probe (Catalog #: FG0165)
  • PTEN/CEN10p FISH Probe (Catalog #: FG0019)
  • TOP2A/CEN17q FISH Probe (Catalog #: FG0119)
Cell Staining Dyes
  • Calcein AM (Catalog #: U0333)
  • DAPI (Catalog #: U0331)
  • Hoechst 33342 (Catalog #: U0334)
  • Propidium Iodide (Catalog #: U0332)

  • Bottle and Tubing Pack (Catalog #: KA4442)
  • CytoQuest™ Single Cell Micromanipulator Set (Catalog #: KA4584)
  • Cell Picking Microscope, Camera, and Software (Catalog #: MR0005)
  • Scanning Microscope and CytoView™ Software (Catalog #: M0019)
  • Blood Collection Tube (Catalog #: MR0006)
Application Notes

Capture and Isolation of EpCAM and CD133 Positive Cells in Peripheral Blood of Non-Small Cell Lung Cancer Patients
Detection of Circulating Tumor Cells in Cholangiocarcinoma Patients
EpCAM CK Positive Circulating Tumor Cells and B7-H3 CD105 Positive Cancer Stem Cells in Clear Cell Renal Cell Carcinoma
Circulating Tumor Cells as Metastatic Biomarker in Oral Squamous Cell Carcinoma
Prenatal Detection of Fetal Trophoblasts in Maternal Circulation
Circulating Epithelial Cells in Dialysis Patient with Acquired Cystic Kidney Disease
Androgen Receptor Gene Amplification in Circulating Tumor Cells of Metastatic Castration-Resistant Prostate Cancer Patient
PSMA vs EpCAM Antibody Capture of Circulating Tumor Cells in Metastatic Castration-Resistant Prostate Cancer Patient
Antibodies Targeting HLA-G and PanCK for Capture and Identification of Circulating Choriocarcinoma Cells
Antibodies Targeting ASGPR and PanCK for Capture and Identification of Circulating Hepatocellular Carcinoma Cells
Plastin-3 Transformed Circulating Tumor Cells in Hepatic Portal Vein of Pancreatic Cancer Patient for Predicting Liver Metastasis
Cell-Surface Vimentin as Novel Epithelial-Mesenchymal Transition and Cancer Stem Cell Biomarkers for Pancreatic Cancer Circulating Tumor Cells
Cell-Surface Vimentin Transformed EMT CTCs and CSCs in Metastatic Castration-Resistant Prostate Cancer
Cell-Surface Vimentin and Pan Cytokeratin Positive Circulating Tumor Cells in Pleural Effusion of Lung Adenocarcinoma Patient
CDX2 Intestinal-Specific Expression in Circulating Tumor Cells of Metastatic Colorectal Adenocarcinoma Patient
Detection of CD44v9 EMT and CSC Protein Biomarker in Circulating Tumor Cells of Castration-Resistant Prostate Cancer Patient
GFAP and EGFRvIII Detection of Glioblastoma Multiforme Circulating Tumor Cells Captured by Cell-Surface Vimentin Antibody
Isolation of DBA Positive Circulating Cancer Stem Cells in Glioblastoma Multiforme
Compare EpCAM PanCK and CSV CSV Antibody Capture and Detection of Ovarian Papillary Serous Cystadenocarcinoma
Cell-Surface Vimentin Antibody for Postive Microfluidic Enrichment and Enumeration of Neuroblastoma CTCs and DTCs
PD-L1 Expression in Cell-Surface Vimentin Positive Circulating Tumor Cells of Colorectal Cancer Patient
EGFR L858R and Ex19wt In Situ mutaFISH™ Detection in Circulating Tumor Cells of Non-Small Cell Lung Cancer








CytoQuest™ CR - Advanced Microfluidic System for Capture, Enumeration, Isolation, and Release of Circulating Tumor Cells
CytoQuest™ CR - Diverse Clinical Applications of Circulating Tumor Cells in Cancers





  CytoQuest™ CR - Circulating Rare Cell Positive Enrichment & Retrieval System
  CytoQuest™ CR Cell Kits & Bioreagents
  Epithelial-Mesenchymal Transition Antibody - Plastin-3
  Epithelial Stem Cell Monoclonal Antibodies - CD44 Variant Isoforms
  Cell-Surface Vimentin (CSV) Monoclonal Antibody
  Cell-Surface Vimentin (CSV) Antibody Capture of Circulating Tumor Cells in Sarcoma Patients
  mutaFISH™ - mutation-specific Fluorescence In Situ Hybridization
  Cancers & Cell-Surface Vimentin CTC Clinical Applications
  FDA-Approved Drugs in Cancer Immunotherapy
  Therapeutic Advances in Immune Checkpoint Inhibitors



User Manual


  Please inquire





  CytoQuest™ CR Software v3.2.
  CytoQuest™ CR Software v3.3.
        Download and decompress zip file to desktop. Run the executable file directly in the folder.



  1. CytoQuest™ CR Unpacking
  2. CytoQuest™ CR Installation
  3. CytoQuest™ CR CTC Leucosep Preparation
  4. CytoQuest™ CR CTC Automated Leucosep Preparation
  5. CytoQuest™ CR CTC Capture Antibody Immobilization
  6. CytoQuest™ CR CTC Capture
  7. CytoQuest™ CR CTC Immunostaining
  8. CytoQuest™ CR CTC FISH
  9. CytoQuest™ CR System Maintenance
  10. CytoQuest™ CR SCx™ Spiral Chamber Assembly - Dos and Don'ts
  11. CytoQuest™ CR Plugs Assembly and CytoChipNano Placement - Dos and Don'ts
  12. CytoQuest™ CR CTC Capture and CTC FISH - Dos and Don'ts
  13. CytoQuest™ CR Troubleshooting I (Access Required)
  14. CytoQuest™ CR Troubleshooting II (Access Required)
  15. CytoQuest™ CR Troubleshooting III (Access Required)
  16. CytoQuest™ CR Troubleshooting IV (Access Required)


CytoQuest™ CR system, SCx™ spiral chamber, TBx™ tension balancer, HBx™ micromixer, and TCx™ thermal control are trademarks and pending patents of Abnova Corporation.

Cell-Surface Vimentin (CSV) antibody is a pending MD Anderson patent which has been exclusively licensed to Abnova Corporation.

mutaFISH™ is a pending trademark and patent of Abnova Corporation.

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