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Last updated: 2016/12/4
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CytoQuest™ CR - Circulating Rare Cell Positive Enrichment & Retrieval System

Abnova’s CytoQuest™ CR is a non-invasive system for capture, enumeration, isolation and retrieval of circulating rare cells (CRCs). Three major subtypes of CRCs in translational research and clinical studies are circulating tumor cells (CTCs), circulating progenitor cells (CPCs), and circulating fetal cells (CFCs). A challenge for market adoption of CRCs is the efficient and reproducible identification, single cell isolation, and retrieval of highly pure and viable CRCs, with their applications supported by a wide repertoire of standardized, GMP grade bioreagents.

CytoQuest™ CR technology utilizes SCx™ spiral chamber, HBx™ micromixer, antibody immobilized nanoarray, and TCx™ thermal control to enable a multitude of CRC functions. SCx™ spiral chamber is equipped with a non-sticky coil and a self-contained micro-vibrator for unimpeded delivery of pretreated blood sample into the nanoarray. HBx™ micromixer provides a herringbone conduit for cell mixing. CytoChipNano is a streptavidin nanoarray which captures the CRCs for cell enumeration and single cell isolation by laser microdissection or micromanipulation. CytoChipNano CR is a specialized streptavidin nanoarray with thermo-sensitive coating which captures and releases the CRCs via a TCx™ thermal control of alternating temperatures.

Monoclonal antibodies targeting specific surface biomarkers of circulating rare cells can be flexibly interchanged to accommodate the biomarker switch in CRC progression. This results in highly pure and viable CRCs for downstream protein characterizations, gene analyses, and cell assays. Cell enumeration is currently the only FDA approved application as a prognostic marker in breast, prostate, and colorectal cancers. In contrast, effective single cell isolation and retrieval of CRCs opens up new scope of applications in the diagnostic and pharmaceutical industry.


 
Catalog # : M0014-04
Price: Please Inquire
Regulatory Status : CE-IVD
 
Subtypes of CRCs

  • Circulating Tumor Cells (CTCs)
  • Circulating Progenitor Cells (CPCs)
  • Circulating Fetal Cells (CFCs)
 
Applications of CTCs

Protein Characterizations
  • Immunofluorescence
  • Proximity ligation assay (PLA)
Gene Analyses
  • Fluorescent In Situ Hybridization (FISH)
  • Polymerase Chain Reaction (PCR)
      Gene Amplifications (GAs)
      Somatic Mutations (SMs)
      Single Nucleotide Polymorphisms (SNPs)
  • Comparative Genomic Hybridization (CGH)
      Copy Number Variants (CNVs)
  • Next Generation Sequencing (NGS)
Cell Assays
  • Primary cell line construction
  • Compound screening
 
Advantages of CytoQuest™ CR Platform

Integrated system
  • Bottle reservoir
  • Fluidic handler
  • SCx™ spiral chamber
  • TBx™ tension balancer
  • Coupler
  • HBx™ micromixer
  • Chip receptor
  • TCx™ thermal chuck
  • Touch user interface
Antibody-based, cell capture
  • Cell enumeration (CytoChipNano)
  • Single cell isolation (CytoChipNano)
  • Thermal cell release (CytoChipNano CR)
 

CytoQuest™ CR                     

Buffer Reservoir
Bottle Reservoir
Fluidic
Fluidic Handler
Chamber
SCx™ Spiral
Chamber
Spiral Chamber Holder
Spiral Chamber Holder
 
TBx™ Tension Balancer
TBx™ Tension Balancer
Coupler
Coupler
Chip Receptor
Chip Receptor
Micromixer
HBx™ Micromixer
 
CytoChipNano
CytoChipNano
CytoChipNano CR
CytoChipNano
CR
TCx™ Thermal Chuck
TCx™ Thermal
Chuck
Chip Opener
Chip Opener
 
Temperature
Temperature Display
QR Code Scanner
QR Code Scanner
Power On LED
Power On LED
Touch User Interface
Touch User Interface
 
 
Hardware General Specifications

Dimensions: 565.06mm(h) x 340.8mm(d) x 271.8mm(w)
Weight: 18 kg
Power: Fluidic Handler: 100-240VAC, 50/60Hz,1.6A
Thermal Controller: 100-240VAC, 50/60Hz, 4.0A
Replace Fuse: Fluidic Handler: 1.6A(Peak)/250V
Thermal Controller: 4A (Peak)/250V
Operating Temperature: 60°F to 104°F (15°C to 40°C)
Programable Thermo Control Profile: 4-40°C, Variable Time Span
 
Hardware Environmental Specifications

Storage Temperature: -5°F to 158°F (-20°C to 70°C)
Operation Altitude: Up to 2000m
Operation Environment: For indoor use
Operating Humidity: 20% to 90% Relative Humidity, Non-Condensing
Storage Humidity: 20% to 95% Relative Humidity, Non-Condensing
 
Software Specifications

Scanner Function: Scanning Capability to Read QR Code/Barcode
Status Display: Progress and Fluid Handling
System Clean: Prime and Clean
Report Generation: Print and Export File
 
Touchscreen Laptop Specifications

Operating System: Windows 7/8/10 (32-bit and 64-bit)
Processor: Pentium III/Celeron 866 MHz or Equivalent
Memory: 1GB Minimum (2GB Recommended)
Free Disk Space: 4GB
Interfaces/USB Ports: USB 2.0 Port*1
 
 
Chip

Cell Enumeration
  • CytoChipNano Combo Pack (Catalog #: KA4440)
  • CytoChipNano (Catalog #: U0095)
  • SCx™ Spiral Chamber (Catalog #: U0314)
  • Plugs (Catalog #: KA4441)
 
Single Cell Isolation
  • CytoChipNano Combo Pack (Catalog #: KA4440)
  • CytoChipNano (Catalog #: U0095)
  • SCx™ Spiral Chamber (Catalog #: U0314)
  • Plugs (Catalog #: KA4441)
 
Capture & Release
  • CytoChipNano CR Combo Pack (Catalog #: KA4862)
  • CytoChipNano CR (Catalog #: U0096)
  • SCx™ Spiral Chamber (Catalog #: U0314)
  • Plugs (Catalog #: KA4441)
 
 
Bioreagent

Circulating Epithelial Cells
  • CytoQuest™ Epithelial Cell Kit (Catalog #: KA4439)
  • CytoQuest™ Epithelial Cell Release Kit (Catalog #: KA4863)
  • CytoQuest™ Epithelial Cell Kit for Mouse Model (Catalog #: KA4576)
  • CytoQuest™ Epithelial Cell Solution (Catalog #: KA3967)
  • CytoQuest™ Plastin-3 Transformed Circulating Epithelial Cell Kit (Catalog #: KA4586)
  • CytoQuest™ CSV Transformed Circulating Epithelial Cell Kit (Catalog #: KA4817)
  • CytoQuest™ CD44v9 Transformed Circulating Epithelial Cell Kit (Catalog #: KA4897)
 
Circulating Tumor Cells
  • CytoQuest™ Choriocarcinoma Cell Kit (Catalog #: KA4530)
  • CytoQuest™ Colorectal Cancer Cell Kit (Catalog #: KA4785)
  • CytoQuest™ Hepatocellular Carcinoma Cell Kit (Catalog #: KA4573)
  • CytoQuest™ Lung Adenocarcinoma Cell Kit (Catalog #: KA4896)
  • CytoQuest™ Pancreatic Cancer Cell Kit (Catalog #: KA4575)
  • CytoQuest™ Prostate Cancer Cell Kit (Catalog #: KA4574)
  • CytoQuest™ Sarcoma Cell Kit (Catalog #: KA4864)
  • CytoQuest™ Universal Circulating Tumor Cell Kit (Catalog #: KA4818)
 
Circulating Cancer Stem Cells
  • CytoQuest™ Clear Cell Renal Cancer Stem Cell Kit (Catalog #: KA4577)
  • CytoQuest™ Pancreatic Cancer Stem Cell Kit (Catalog #: KA4866)
  • CytoQuest™ Universal Cancer Stem Cell Kit (Catalog #: KA4819)
 
Circulating Trophoblasts
  • CytoQuest™ Trophoblast Kit (Catalog #: KA4515)
 
Blockers, Enhancers & Pickers
  • CytoQuest™ FcR Blocking Reagent (Catalog #: U0318)
  • CytoQuest™ MicroBlocker (Catalog #: U0335)
  • CytoQuest™ Staining Blocker (Catalog #: U0330)
  • CytoQuest™ SuperBlocker (Catalog #: U0317)
  • CytoQuest™ Single Cell Picking Kit (Catalog #: KA4578)
 
Gene Amplification, Translocation, and Split FISH Probes
  • ACTN4 DNA Probe (Catalog #: DF0001)
  • AR/CENXp FISH Probe (Catalog #: FG0163)
  • BRCA1/CEN17q FISH Probe (Catalog #: FG0069)
  • CD274 (PD-L1)/CEN9q FISH Probe (Catalog #: FG0160)
  • CEP8 (R6G) FISH Probe (Catalog #: FC0197)
  • EGFR/CEN7p FISH Probe (Catalog #: FG0114)
  • EML4/ALK Translocation FISH Probe (Catalog #: FT0009)
  • ERBB2 (HER2)/CEN17q FISH Probe (Catalog #: FG0166)
  • ERG Split FISH Probe (Catalog #: FS0008)
  • MYC/CEN8q FISH Probe (Catalog #: FG0117)
  • p53/CEN17q FISH Probe (Catalog #: FG0011)
  • PLS3/CENXp FISH Probe (Catalog #: FG0164)
  • PRDM14 FISH Probe (Catalog #: FG0165)
  • PTEN/CEN10p FISH Probe (Catalog #: FG0019)
  • TOP2A/CEN17q FISH Probe (Catalog #: FG0119)
 
Cell Staining Dyes
  • Calcein AM (Catalog #: U0333)
  • DAPI (Catalog #: U0331)
  • Hoechst 33342 (Catalog #: U0334)
  • Propidium Iodide (Catalog #: U0332)
 
 
Accessory

  • Bottle and Tubing Pack (Catalog #: KA4442)
  • CytoQuest™ Single Cell Micromanipulator Set (Catalog #: KA4584)
 
  Recommended Microscope
  Recommended Camera
  Heparin Tube Specification
 
 
Application Notes

Capture and Isolation of EpCAM and CD133 Positive Cells in Peripheral Blood of Non-Small Cell Lung Cancer Patients
Detection of Circulating Tumor Cells in Cholangiocarcinoma Patients
EpCAM CK Positive Circulating Tumor Cells and B7-H3 CD105 Positive Cancer Stem Cells in Clear Cell Renal Cell Carcinoma
Circulating Tumor Cells as Metastatic Biomarker in Oral Squamous Cell Carcinoma
Prenatal Detection of Fetal Trophoblasts in Maternal Circulation
Circulating Epithelial Cells in Dialysis Patient with Acquired Cystic Kidney Disease
Androgen Receptor Gene Amplification in Circulating Tumor Cells of Metastatic Castration-Resistant Prostate Cancer Patient
PSMA vs EpCAM Antibody Capture of Circulating Tumor Cells in Metastatic Castration-Resistant Prostate Cancer Patient
Antibodies Targeting HLA-G and PanCK for Capture and Identification of Circulating Choriocarcinoma Cells
Antibodies Targeting ASGPR and PanCK for Capture and Identification of Circulating Hepatocellular Carcinoma Cells
Plastin-3 Transformed Circulating Tumor Cells in Hepatic Portal Vein of Pancreatic Cancer Patient for Predicting Liver Metastasis
Cell-Surface Vimentin as Novel Epithelial-Mesenchymal Transition and Cancer Stem Cell Biomarkers for Pancreatic Cancer Circulating Tumor Cells
Cell-Surface Vimentin Transformed EMT CTCs and CSCs in Metastatic Castration-Resistant Prostate Cancer
Cell-Surface Vimentin and Pan Cytokeratin Positive Circulating Tumor Cells in Pleural Effusion of Lung Adenocarcinoma Patient
CDX2 Intestinal-Specific Expression in Circulating Tumor Cells of Metastatic Colorectal Adenocarcinoma Patient
Detection of CD44v9 EMT and CSC Protein Biomarker in Circulating Tumor Cells of Castration-Resistant Prostate Cancer Patient

 

 

 
Publications

 

 
Support

Posters

 

CytoQuest™ CR - Advanced Microfluidic System for Capture, Enumeration, Isolation, and Release of Circulating Tumor Cells
CytoQuest™ CR - Diverse Clinical Applications of Circulating Tumor Cells in Cancers

 

 

Flyers

 

  CytoQuest™ CR - Circulating Rare Cell Positive Enrichment & Retrieval System
  CytoQuest™ CR Cell Kits & Bioreagents
  Epithelial-Mesenchymal Transition Antibody - Plastin-3
  Epithelial Stem Cell Monoclonal Antibodies - CD44 Variant Isoforms
  Cell-Surface Vimentin (CSV) Monoclonal Antibody
  Cell-Surface Vimentin (CSV) Antibody Capture of Circulating Tumor Cells in Sarcoma Patients

 

 

User Manual

 

  Please inquire us

 

 

Software

 

  CytoQuest CR v3.1.
        Download and decompress zip file to desktop. Run the executable file directly in the folder.

 

 

AbVideo™
  1. CytoQuest™ CR Unpacking
  2. CytoQuest™ CR Installation
  3. CytoQuest™ CR CTC Leucosep Preparation
  4. CytoQuest™ CR CTC Automated Leucosep Preparation
  5. CytoQuest™ CR CTC Capture Antibody Immobilization
  6. CytoQuest™ CR CTC Capture
  7. CytoQuest™ CR CTC Immunostaining
  8. CytoQuest™ CR CTC FISH
  9. CytoQuest™ CR System Maintenance
  10. CytoQuest™ CR SCx™ Spiral Chamber Assembly - Dos and Don'ts
  11. CytoQuest™ CR Plugs Assembly and CytoChipNano Placement - Dos and Don'ts
  12. CytoQuest™ CR CTC Capture and CTC FISH - Dos and Don'ts
  13. CytoQuest™ CR Troubleshooting I (Access Required)
  14. CytoQuest™ CR Troubleshooting II (Access Required)
  15. CytoQuest™ CR Troubleshooting III (Access Required)
  16. CytoQuest™ CR Troubleshooting IV (Access Required)

 

CytoQuest™ CR system, SCx™ spiral chamber, TBx™ tension balancer, HBx™ micromixer, and TCx™ thermal control are trademarks and pending patents of Abnova Corporation.

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