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Last updated: 2017/12/10

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mutaFISH™ RNA Accessory Kit

  • Catalog # : KA4915
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  • Specification
  • Product Description:
  • mutaFISH™ RNA Accessory Kit provides reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
  • Supplied Product:
  • Box 1 content:

    1. DEPC-H2O
    2. DEPC-PBS
    3. PBS-T
    4. Permeabilization Buffer
    5. Formamide
    6. Detection Buffer


    Box 2 content:

    1. 3% Paraformaldehyde
    2. dNTP Mix
    3. BSA
    4. RNase Inhibitor
    5. RT Enzyme
    6. 5X RT Buffer
    7. Hybrid Enzyme
    8. 10X Hybrid Enzyme Buffer
    9. RNase H
    10. 1M KCL
    11. DNA Polymerase
    12. 10X DNA Polymerase Buffer
    13. 50% Glycerol
    14. DAPI (100uM)
  • Storage Instruction:
  • Store Box 1 at 4°C, store Box 2 at -20°C.
  • Regulatory Status:
  • For research use only (RUO)
  • Applications
  • mutation specific, Fluorescence In Situ Hybridization
  • mutation specific, Fluorescence <em>In Situ</em> Hybridization
  • The figure represents an example workflow of detecting a point mutation on single strand RNA using mutaFISH™ probes with mutaFISH™ RNA Accessory Kit.
  • Application Image
  • mutation specific, Fluorescence In Situ Hybridization
  • mutation specific, Fluorescence <em>In Situ</em> Hybridization
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