mutaFISH™ CK7wt RNA Probes
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Specification
Product Description
mutaFISH™ CK7wt RNA Probes is designed to detect human CK7 gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. RT CK7 Primer
2. mutaFISH™ CK7 RNA Probe
3. Detection Probe-FITCComparison
Fluorophore
FITC (Excitation Peak (nm): 495; Emission Peak (nm): 519)
Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human HeLa cells. CK7 gene was detected via green signal (FITC).mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
mutaFISH™ staining was performed in situ in mouse FFPE CK7 HeLa tissue. CK7 gene was detected via green signal (FITC). -
Gene Info — KRT7
Entrez GeneID
3855Gene Name
KRT7
Gene Alias
CK7, K2C7, K7, MGC129731, MGC3625, SCL
Gene Description
keratin 7
Omim ID
148059Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq
Other Designations
cytokeratin 7|keratin, 55K type II cytoskeletal|keratin, simple epithelial type I, K7|keratin, type II cytoskeletal 7|sarcolectin|type II mesothelial keratin K7
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