mutaFISH™ IDH1 R132H R132wt IDH2 R172K R172wt RNA Probes
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More Files
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mutation specific, Fluorescence In Situ Hybridization (Cells)
Fig.1 mutaFISH™ staining was performed in situ in human SH-SY5Y cells. IDH1 R123H point mutation was not detected via orange signal (Cy3), IDH1 R123 wildtype was detected via red signal.
Fig.2 mutaFISH™ staining was performed in situ in human SH-SY5Y cells. IDH2 R172K point mutation was detected via purple signal (Texas Red), IDH2 R172 wildtype was detected via red signal.
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Specification
Product Description
mutaFISH™ IDH1 R132H R132wt IDH2 R172K R172wt RNA Probes is designed to detect human IDH1 R132H gene mutation and IDH2 R172K gene mutation on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. RT IDH1 R132 Primer
2. RT IDH2 R172 Primer
3. mutaFISH™ IDH1 R132H RNA Probe
4. mutaFISH™ IDH1 R132wt RNA Probe
5. mutaFISH™ IDH2 R172K RNA Probe
6. mutaFISH™ IDH2 R172wt RNA Probe
7. Detection Probe-Aqua 431
8. Detection Probe-Texas Red X
9. Detection Probe-6-HEXComparison
Fluorophore
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)
Texas Red X (Excitation Peak (nm): 595; Emission Peak 613)
6-HEX (Excitation Peak (nm): 533; Emission Peak (nm): 559)Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
Fig.1 mutaFISH™ staining was performed in situ in human SH-SY5Y cells. IDH1 R123H point mutation was not detected via orange signal (Cy3), IDH1 R123 wildtype was detected via red signal.
Fig.2 mutaFISH™ staining was performed in situ in human SH-SY5Y cells. IDH2 R172K point mutation was detected via purple signal (Texas Red), IDH2 R172 wildtype was detected via red signal. -
Gene Info — IDH1
Entrez GeneID
3417Gene Name
IDH1
Gene Alias
IDCD, IDH, IDP, IDPC, PICD
Gene Description
isocitrate dehydrogenase 1 (NADP+), soluble
Omim ID
147700Gene Ontology
HyperlinkGene Summary
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq
Other Designations
NADP+-specific ICDH|NADP-dependent isocitrate dehydrogenase, cytosolic|NADP-dependent isocitrate dehydrogenase, peroxisomal|oxalosuccinate decarboxylase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glutathione metabolism
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Disease
- Adenoma
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- Blast Crisis
- Brain Neoplasms
- Carcinoma
- Chronic Disease
- Cleft Lip
- Cleft Palate
- Disease Progression
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