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CytoQuest™ Plastin-3 EMT Transformed Circulating Epithelial Cell Kit

  • Catalog # : KA4586
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  • Specification
  • Product Description:
  • CytoQuest™ Plastin-3 EMT Transformed Circulating Epithelial Cell Kit contains antibodies for immobilization and immunostaining of EMT transformed circulating epithelial cells.
  • Supplied Product:
  • Kit content:
    1. Anti-EpCAM capturing antibody (Biotin):
    Biotin conjugated Anti-EpCAM antibody for EMT transformed circulating epithelial cell capturing.

    2. Anti-PanCK detecting antibody (FITC):
    FITC conjugated Anti-PanCK antibody for EMT transformed circulating epithelial cell detection.

    3. Anti-Plastin-3 detecting antibody (Alexa 647):
    Alexa 647 conjugated Anti-Plastin-3 antibody for EMT transformed circulating epithelial cell detection.

    4. Anti-CD45 detecting antibody (PE):
    PE conjugated Anti-CD45 antibody for EMT transformed circulating epithelial cell detection.

    5. 50X Antibody Dilution Buffer (50X ADB).

    *Reagents are sufficient for 20 assays using recommended protocol.
  • Storage Instruction:
  • Store Anti-EpCAM capturing antibody (Biotin), Anti-PanCK detecting antibody (FITC), Anti-Plastin-3 detecting antibody (Alexa 647) and Anti-CD45 detecting antibody (PE) at 4°C.

    Store 50X Antibody Dilution Buffer (50X ADB) at -20°C.

    Aliquot to avoid repeated freezing and thawing.
  • Regulatory Status:
  • For research use only (RUO)
  • Applications
  • Immunofluorescence (Circulating Tumor Cell)
  • Immunofluorescence (Circulating Tumor Cell)
  • Representative images of CTC (white arrow) and WBCs (yellow arrow) from pancreatic cancer patient. CTCs was detected by using immunofluorescence staining for PanCK (FITC, green), Plastin-3 (Alexa647, red), CD45 (PE, orange) and Nucleus (DAPI, blue).
  • Application Image
  • Immunofluorescence (Circulating Tumor Cell)
  • Immunofluorescence (Circulating Tumor Cell)
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