CytoQuest™ EpCAM PanCK CD45 PLS3 EMT Antibody Kit
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More Files
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Immunofluorescence (Circulating Tumor Cell)
Representative images of CTC (white arrow) and WBCs (yellow arrow) from pancreatic cancer patient. CTCs was detected by using immunofluorescence staining for PanCK (FITC, green), Plastin-3 (Alexa647, red), CD45 (PE, orange) and Nucleus (DAPI, blue).
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Specification
Product Description
CytoQuest™ EpCAM PanCK CD45 PLS3 EMT Antibody Kit contains antibodies for immobilization and immunostaining of EMT transformed circulating epithelial cells.
Instrument Requirement
Chip Requirement
Supplied Product
Kit content:
1. Anti-EpCAM capturing antibody (Biotin):
Biotin conjugated Anti-EpCAM antibody for EMT transformed circulating epithelial cell capturing.
2. Anti-PanCK detecting antibody (FITC):
FITC conjugated Anti-PanCK antibody for EMT transformed circulating epithelial cell detection.
3. Anti-Plastin-3 detecting antibody (Alexa 647):
Alexa 647 conjugated Anti-Plastin-3 antibody for EMT transformed circulating epithelial cell detection.
4. Anti-CD45 detecting antibody (PE):
PE conjugated Anti-CD45 antibody for EMT transformed circulating epithelial cell detection.
5. 50X Antibody Dilution Buffer (50X ADB).
*Reagents are sufficient for 20 assays using recommended protocol.Regulatory Status
For research use only (RUO)
Storage Instruction
Store Anti-EpCAM capturing antibody (Biotin), Anti-PanCK detecting antibody (FITC), Anti-Plastin-3 detecting antibody (Alexa 647) and Anti-CD45 detecting antibody (PE) at 4°C.
Store 50X Antibody Dilution Buffer (50X ADB) at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence (Circulating Tumor Cell)
Representative images of CTC (white arrow) and WBCs (yellow arrow) from pancreatic cancer patient. CTCs was detected by using immunofluorescence staining for PanCK (FITC, green), Plastin-3 (Alexa647, red), CD45 (PE, orange) and Nucleus (DAPI, blue).
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