Immunofluorescence staining on non-fixed, non-permeabilized MDA-MB-231 cells using FITC conjugated Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1 for CSV (Green) and DAPI for nucleus (Blue).
Immunofluorescence staining of CSV on isolated LM7 cancer cells and leukocytes using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. The cells were co-stained against DRAQ5, CSV and CD45. (Satelli A., et al. Cancer Research 2014; 74:1645-1650)
Flow cytometric analysis of CSV expression in cancer (top) and normal (bottom) cell lines using Cell-Surface Vimentin (CSV) monoclonal antibody, clone 84-1. Isotype controls were used as negative controls. (Satelli A., et al., Clinical Cancer Research 2014; 21(4):899-906)
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract