mutaFISH™ EML4wt ALKwt RNA Probes
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More Files
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mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human H1975 cells. EML4 was detected via purple signal (Texas Red); ALK was not detected.
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Specification
Product Description
mutaFISH™ EML4wt ALKwt RNA Probes is designed to detect human EML4 and ALK gene rearrangement on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. RT EML4 Primer
2. RT ALK Primer
3. mutaFISH™ EML4wt RNA Probe
4. mutaFISH™ ALKwt RNA Probe
5. Detection Probe-6-HEX
6. Detection Probe-Texas Red XComparison
Fluorophore
6-HEX (Excitation Peak (nm): 533; Emission Peak (nm): 559)
Texas Red X (Excitation Peak (nm): 595; Emission Peak 613)Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human H1975 cells. EML4 was detected via purple signal (Texas Red); ALK was not detected. -
Gene Info — ALK
Entrez GeneID
238Gene Name
ALK
Gene Alias
CD246, Ki-1, TFG/ALK
Gene Description
anaplastic lymphoma receptor tyrosine kinase
Omim ID
105590Gene Ontology
HyperlinkGene Summary
The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeq
Other Designations
ALK tyrosine kinase receptor|CD246 antigen|anaplastic lymphoma kinase (Ki-1)|anaplastic lymphoma kinase Ki-1
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Gene Info — EML4
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Interactome
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Disease
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