CytoQuest™ EpCAM PanCK CD45 CSV EMT Antibody Kit
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Product Description
CytoQuest™ EpCAM PanCK CD45 CSV EMT Antibody Kit contains antibodies for immobilization and immunostaining of EMT transformed circulating epithelial cells.
Instrument Requirement
Chip Requirement
Supplied Product
Kit content:
1. Anti-EpCAM capturing antibody (Biotin):
Biotin conjugated Anti-EpCAM antibody for EMT transformed circulating epithelial cell capturing.
2. Anti-PanCK detecting antibody (FITC):
FITC conjugated Anti-PanCK antibody for EMT transformed circulating epithelial cell detection.
3. Anti-CSV detecting antibody (APC):
APC conjugated Anti-CSV antibody for EMT transformed circulating epithelial cell detection.
4. Anti-CD45 detecting antibody (PE):
PE conjugated Anti-CD45 antibody for EMT transformed circulating epithelial cell detection.
5. 50X Antibody Dilution Buffer (50X ADB).
*Reagents are sufficient for 20 assays using recommended protocol.Regulatory Status
For research use only (RUO)
Storage Instruction
Store Anti-EpCAM capturing antibody (Biotin), Anti-PanCK detecting antibody (FITC), Anti-CSV detecting antibody (APC) and Anti-CD45 detecting antibody (PE) at 4°C.
Store 50X Antibody Dilution Buffer (50X ADB) at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Cell-Surface Vimentin (CSV) detecting antibody is best used before cell fixation and permeabilization. If fixation is required, please use Abnova’s Special Fixative.
Cell-Surface Vimentin (CSV) antibody is a pending MD Anderson patent which has been exclusively licensed to Abnova Corporation. -
Applications
Immunofluorescence (Circulating Tumor Cell)
Representative images of EMT transformed CTCs (white arrow) and WBC from pancreatic cancer patient. CSV EMT CTCs were detected by using immunofluorescence staining for CSV (FITC, green), CD45 (PE, orange) and Nucleus (DAPI, blue).
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