mutaFISH™ BRAF V600E V600wt RNA Probes
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More Files
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mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human Sk-HEP1 cells. BRAF V600E point mutation was detected via orange signal (Cy3), BRAF V600wt was detected via red signal (Cy5).
mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
mutaFISH™ staining was performed in situ in mouse FFPE BRAF 293T tissue. BRAF V600wt was detected via red signal (Texas Red X).
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Specification
Product Description
mutaFISH™ BRAF V600E V600wt RNA Probes is designed to detect human BRAF V600E gene mutation on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. RT BRAF V600 Primer
2. mutaFISH™ BRAF V600E RNA Probe
3. mutaFISH™ BRAF V600wt RNA Probe
4. Detection Probe-Aqua 431
5. Detection Probe-Texas Red XComparison
Fluorophore
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)
Texas Red X (Excitation Peak (nm): 595; Emission Peak 613)Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human Sk-HEP1 cells. BRAF V600E point mutation was detected via orange signal (Cy3), BRAF V600wt was detected via red signal (Cy5).mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
mutaFISH™ staining was performed in situ in mouse FFPE BRAF 293T tissue. BRAF V600wt was detected via red signal (Texas Red X). -
Gene Info — BRAF
Entrez GeneID
673Gene Name
BRAF
Gene Alias
B-RAF1, BRAF1, FLJ95109, MGC126806, MGC138284, RAFB1
Gene Description
v-raf murine sarcoma viral oncogene homolog B1
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
Other Designations
94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|Murine sarcoma viral (v-raf) oncogene homolog B1
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Interactome
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Pathway
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Disease
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