mutaFISH™ EGFR L858R L858wt Ex19wt RNA Probes
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mutation specific, Fluorescence In Situ Hybridization (Cells)
Fig.1 mutaFISH™ staining was performed in situ in human H1975 cells and with EpCAM immunostained via FITC. EGFR L858R point mutation was detected via orange signal (Cy3), EGFR L858wt was detected via red signal (Cy5), and EGFR Ex19wt was detected via green signal (FITC).
Fig. 2 mutaFISH™ staining was performed in situ in human PC-14 cells (white arrow) and with PanCK immunostained via FITC. EGFR L858R point mutation was not detected, EGFR L858wt was detected via red signal (Cy5), and EGFR Ex19wt was not detected.
mutation specific, Fluorescence In Situ Hybridization (Frozen Tissue)
mutaFISH™ staining was performed in situ in human frozen tissue from lung adenocarcinoma. EGFR L858R point mutation was detected via orange signal (Cy3), EGFR L858wt was detected via red signal (Cy5), and EGFR Ex19wt was detected via green signal (Texas Red).
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Specification
Product Description
mutaFISH™ EGFR L858R L858wt Ex19wt RNA Probes is designed to detect human EGFR L858R gene mutation and EGFR exon 19 deletion on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Reactivity
Human
Supplied Product
Content:
1. RT EGFR L858 Primer
2. RT EGFR Ex19 Primer
3. mutaFISH™ EGFR L858R RNA Probe
4. mutaFISH™ EGFR L858wt RNA Probe
5. mutaFISH™ EGFR Ex19wt RNA Probe
6. Detection Probe-Aqua 431
7. Detection Probe-Texas Red X
8. Detection Probe-6-HEXComparison
Fluorophore
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)
Texas Red X (Excitation Peak (nm): 595; Emission Peak 613)
6-HEX (Excitation Peak (nm): 533; Emission Peak (nm): 559)Probe Position
Regulatory Status
For research use only (RUO)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Video
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
Fig.1 mutaFISH™ staining was performed in situ in human H1975 cells and with EpCAM immunostained via FITC. EGFR L858R point mutation was detected via orange signal (Cy3), EGFR L858wt was detected via red signal (Cy5), and EGFR Ex19wt was detected via green signal (FITC).
Fig. 2 mutaFISH™ staining was performed in situ in human PC-14 cells (white arrow) and with PanCK immunostained via FITC. EGFR L858R point mutation was not detected, EGFR L858wt was detected via red signal (Cy5), and EGFR Ex19wt was not detected.mutation specific, Fluorescence In Situ Hybridization (Frozen Tissue)
mutaFISH™ staining was performed in situ in human frozen tissue from lung adenocarcinoma. EGFR L858R point mutation was detected via orange signal (Cy3), EGFR L858wt was detected via red signal (Cy5), and EGFR Ex19wt was detected via green signal (Texas Red). -
Gene Info — EGFR
Entrez GeneID
1956Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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