CytoQuest™ Checkpoint Circulating Tumor Cell CSV CSV CD45 PD-L1 Antibody Kit
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Product Description
CytoQuest™ Checkpoint Circulating Tumor Cell CSV CSV CD45 PD-L1 Antibody Kit contains antibodies for immobilization and immunostaining of circulating timor cells.
Instrument Requirement
Chip Requirement
Supplied Product
Kit content:
1. Anti-CSV capturing antibody (Biotin):
Biotin conjugated Anti-CSV antibody for circulating tumor cell capturing.
2. Anti-CSV detecting antibody (FITC):
FITC conjugated Anti-CSV antibody for circulating tumor cell detection.
3. Anti-CD45 detecting antibody (PE):
PE conjugated Anti-CD45 antibody for circulating tumor cell detection.
4. Anti-PD-L1 detecting antibody:
Anti-PD-L1 detecting antibody for circulating tumor cell detection.
5. Secondary antibody (Alexa 647)
6. 50X Antibody Dilution Buffer (50X ADB).
*Reagents are sufficient for 20 assays using recommended protocol.Regulatory Status
For research use only (RUO)
Storage Instruction
Store Anti-CSV capturing antibody (Biotin), Anti-CSV detecting antibody (FITC), Anti-CD45 detecting antibody (PE) and Secondary antibody (Alexa647) at 4°C.
Store Anti-PD-L1 detecting antibody and 50X Antibody Dilution Buffer (50X ADB) at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Cell-Surface Vimentin (CSV) detecting antibody is best used before cell fixation and permeabilization. If fixation is required, please use Abnova’s Special Fixative.
Cell-Surface Vimentin (CSV) antibody is a pending MD Anderson patent which has been exclusively licensed to Abnova Corporation. -
Applications
Immunofluorescence (Circulating Tumor Cell)
Representative images of CTC (white arrow)from the patient. CTCs were detected by using immunofluorescence staining for CSV(FITC, green), CD45 (PE, orange), PD-L1 (Alexa647, red) and Nucleus (Hoechst 33342, blue).
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