TNF monoclonal antibody, clone TNF706
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human TNF.
Immunogen
Recombinant protein corresponding to N-terminus of human TNF.
Host
Mouse
Theoretical MW (kDa)
17
Reactivity
Human, Rat
Form
Liquid
Purification
Protein A/G purification
Isotype
IgM, kappa
Recommend Usage
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 45 min a 95°C followed by cooling at RT for 20 minutes)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human Erdheim Chester disease (also known as polyostotic lerosing histiocytosis) and (B) rat pancreas with TNF monoclonal antibody, clone TNF706 (Cat # MAB14620).Immunofluorescence
Immunofluorescence staining of HepG2 cells using this antibody followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with Reddot. -
Gene Info — TNF
Entrez GeneID
7124Protein Accession#
P01375Gene Name
TNF
Gene Alias
DIF, TNF-alpha, TNFA, TNFSF2
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
Other Designations
APC1 protein|OTTHUMP00000029281|OTTHUMP00000037669|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Improvement in sensitivity of enzyme-linked immunosorbent assay for tumour necrosis factor.
P J McLaughlin, N J Elwood, L T Ramadi, M R Pica, I F McKenzie.
Immunology and Cell Biology 1990 Feb; 68 ( Pt 1):51.
Application:ELISA, Recombinant protein.
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Improvement in sensitivity of enzyme-linked immunosorbent assay for tumour necrosis factor.
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