Stable Cell Line Antibody Production

Abnova specializes in the rapid and efficient establishment of stable cell lines that can constitutively express the excellent quality of rabbit, mouse, or human monoclonal antibodies. High efficient expression constructs are transfected and stably integrated into the mammalian 293 or CHO-S cell genome. After multiple post-transfection passages in selection media, high-expression clones from the stably transfected population are then isolated and verified for IgG expression by SDS-PAGE Coomassie Blue staining and ELISA. Subsequent development and optimized growth of these positive clones will be promoted in a serum-free suspension environment. Within 2-4 months, the top three expression cell lines with three vials each will be ready for delivery. Service for antibody production following the generation of the stable cell lines at gram quantities is also available. The actual turnaround time for scale-up antibody production and purification may vary for each cell line.