Mammalian expressed proteins are important bioreagents to accelerate your research. Abnova provides a full-service mammalian protein encompassing cloning, expression, production, and purification based on proprietary expression plasmids, His8-Sumo and IgG1 Fc tags, PEI transfection in HEK293 and CHO cells, and Ni-NTA or Protein A affinity column purification. The purified protein is checked by SDS-PAGE and Western Blot. Customers can also provide downstream assays to confirm the bioactivity of the protein. Our technical support is available to accommodate additional requests and needs required by your experiments. We take care of the proteins so you can focus on your research!

Flowchart

 
 
 

Specification
Mammalian Transient Protein Expression Service Details

 
 

Advantages
  • High efficiency
  • Optimized mammalian expression system
  • High-throughput and large-scale production
  • High yield expression with post-translational modification and correct folding
 
 

Examples
ELISA Result of Human IL-12 Biological Activity
 

 
Measure by its ability to induce IFN gamma secretion in PHA-activated human peripheral blood lymphocytes (PBMC). The ED50 for this effect is 0.05~0.2 ng/mL.
 
 

ELISA Results of HMGB1 Biological Activity
 

 

(A) Human HMGB1 recombinant protein induced TNF alpha with a dose-dependent manner in RAW264.7 cells.
(B) Human HMGB1 C23AC45A mutated recombinant protein induced TNF alpha with lower amount than wild-type HMGB1 protein in RAW264.7 cells.
(C) Human HMGB1 C23AC45AC106A mutated recombinant protein almost not induced TNF alpha in RAW264.7 cells.

For any inquiry, please contact : OEM@abnova.com

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