High Throughput Wheat Germ Protein Expression
Abnova's cell-free protein synthesis system is pioneered by Professor Yaeta Endo of Ehime University, Japan. This high throughput platform is based on the eukaryotic translation apparatus of wheat germ. Wheat embryos store all of the components of translation in a concentrated dried state, ready for protein synthesis as soon as germination starts. Conventional wheat germ extracts contain the RNA N-glycosidase tritin and other inhibitors of translation such as thionin, ribonucleases, deoxyribonucleases, and proteases. These inhibitors originate from the endosperm. Extensive washing of wheat embryos to eliminate endosperm contaminants has resulted in extracts with a high degree of stability and activity. By using mRNA having 5'-cap and a poly(A)-tail with this extract, the translation reaction yields mg quantities of active proteins per ml of reaction volume. This system has significant advantages over other commonly used protein expression systems. With an expression vector designed for large scale protein expression, the platform is amenable to production of many proteins in parallel using robotic instruments. Assessment of the proteins produced by this technology showed many of them retained their correct foldings and biological functions.



  • High Solubility
  • High Yield
  • High Success Rate
  • Automated Expression and Purification
  • More Native Protein Conformation

Protein Types


  • Angiogenesis
  • Apoptosis
  • Binding
  • Cell Ad/Junc/Cytoskel
  • Cell Cycle
  • Cytokine
  • Enzyme
  • Membrane
  • Metabolism
  • Neurobiology
  • Plasma/Serum
  • Signal Transduction
  • Stem Cell
  • Transcriptions
  • Ubiquitin
  • Others


Performance Comparison Among Several Expression Systems

  Wheat Germ Extruct
(in vitro)
(in vivo)
(in vivo)
(in vitro)
Yeast CHO
Coverage Excellent Good Poor Poor Good Good
Folding and Activity Good Good Poor Poor Poor Good
Through-put Excellent Poor Poor Good Poor Poor
Typical process time 1 day 14 days 3-4 days 1 day 3-4 days 14 days
Amino acids labeling Excellent Poor Poor Good Poor Poor
Glycosylation No Insect type No No Yeast type Mammalian type



For any inquiry, please contact : OEM@abnova.com

For any inquiry, please contact
We have placed cookies on your device to make our website better. If you continue using our website, we assume you agree to it. You can learn more details here .