TNF recombinant monoclonal antibody, clone CDP 571
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More Files
- More Functions
Western Blot
Western Blot analysis were resolved on a 10% SDS PAGE gel and blots probed at 1 µg/ml before being detected by a secondary antibody. The expected band size for TNFalpha is 26 kDa, however dimers and other higher order complexes are well described in the literature too successfully detected human and rat TNF alpha.
Immunohistochemistry
Immunohistochemistry analysis of Formalin fixed rat liver slices were stained at 5 ug/mL.
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human TNF alpha.
Antibody Species
Mouse
Immunogen
Original antibody is raised against human TNF-alpha
Reactivity
Human
Specificity
The antibody binds to human TNF-alpha with a Kd of 0.087 nM.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG1, Kappa
Recommend Usage
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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Applications
Western Blot
Western Blot analysis were resolved on a 10% SDS PAGE gel and blots probed at 1 µg/ml before being detected by a secondary antibody. The expected band size for TNFalpha is 26 kDa, however dimers and other higher order complexes are well described in the literature too successfully detected human and rat TNF alpha.Immunohistochemistry
Immunohistochemistry analysis of Formalin fixed rat liver slices were stained at 5 ug/mL. -
Gene Info — TNF
Entrez GeneID
7124Protein Accession#
P01375Gene Name
TNF
Gene Alias
DIF, TNF-alpha, TNFA, TNFSF2
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq
Other Designations
APC1 protein|OTTHUMP00000029281|OTTHUMP00000037669|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
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Interactome
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Pathway
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Disease
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