TOP1 recombinant monoclonal antibody, clone 6D8
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: K562 whole cell lysate; Lane 4: HL60 whole cell lysate; Lane 5: PC-3 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of TOP1 recombinant monoclonal antibody, clone 6D8 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.
Immunoprecipitation
Immunoprecipitating TOP1 in K562 whole cell lysate.
Flow Cytometry
Overlay histogram showing HepG2 cells stained with TOP1 recombinant monoclonal antibody, clone 6D8 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TOP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human TOP1.
Theoretical MW (kDa)
Calculated MW: 91 kD
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation(1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: K562 whole cell lysate; Lane 4: HL60 whole cell lysate; Lane 5: PC-3 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of TOP1 recombinant monoclonal antibody, clone 6D8 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.Immunoprecipitation
Immunoprecipitating TOP1 in K562 whole cell lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing HepG2 cells stained with TOP1 recombinant monoclonal antibody, clone 6D8 (red line) at 1:50. -
Gene Info — TOP1
Entrez GeneID
7150Protein Accession#
P11387Gene Name
TOP1
Gene Alias
TOPI
Gene Description
topoisomerase (DNA) I
Omim ID
126420Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq
Other Designations
DNA topoisomerase I|OTTHUMP00000031713|type I DNA topoisomerase
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Interactome
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Disease
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