TOP1 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TOP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human TOP1.
Theoretical MW (kDa)
90
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Topoisomerase 1 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates with Topoisomerase 1 recombinant monoclonal antibody (Cat # RAB02549).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human colon cancer formalin fixed paraffin embedded tissue section using TOP1 recombinant monoclonal antibody (Cat # RAB02549). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.62). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with TOP1 recombinant monoclonal antibody (Cat # RAB02549). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — TOP1
Entrez GeneID
7150Protein Accession#
P11387Gene Name
TOP1
Gene Alias
TOPI
Gene Description
topoisomerase (DNA) I
Omim ID
126420Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq
Other Designations
DNA topoisomerase I|OTTHUMP00000031713|type I DNA topoisomerase
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