TOP1 recombinant monoclonal antibody, clone R02-2H9
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More Files
- More Functions
Western Blot
Western blot analysis of Topoisomerase I in K562, rat Brain, C6, 3T3, Hela lysates using Topoisomerase I antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TOP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Topoisomerase I.
Theoretical MW (kDa)
Calculated MW: 91 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Topoisomerase I in K562, rat Brain, C6, 3T3, Hela lysates using Topoisomerase I antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
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Gene Info — TOP1
Entrez GeneID
7150Protein Accession#
P11387Gene Name
TOP1
Gene Alias
TOPI
Gene Description
topoisomerase (DNA) I
Omim ID
126420Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq
Other Designations
DNA topoisomerase I|OTTHUMP00000031713|type I DNA topoisomerase
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Interactome
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Disease
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