PCNA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PCNA.
Immunogen
A synthetic peptide corresponding to internal region of human PCNA.
Host
Rabbit
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Monkey, Mouse, Rat
Specificity
This affinity purified antibody is directed against human PCNA protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:8000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using PCNA polyclonal antibody (Cat # PAB10307) shows detection of PCNA protein in HEK293 (Lane 1) and Jurkat (Lane 2) whole cell extracts.
Approximately 25 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose.
After blocking, the membrane was incubated with the primary antibody diluted to 1 : 1000.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye® 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (Lane M, 700 nm channel, red).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PCNA
Entrez GeneID
5111Protein Accession#
NP_872590;P12004Gene Name
PCNA
Gene Alias
MGC8367
Gene Description
proliferating cell nuclear antigen
Omim ID
176740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
Other Designations
DNA polymerase delta auxiliary protein|OTTHUMP00000030189|OTTHUMP00000030190|cyclin
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Interactome
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Publication Reference
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A defined human system that supports bidirectional mismatch-provoked excision.
Dzantiev L, Constantin N, Genschel J, Iyer RR, Burgers PM, Modrich P.
Molecular Cell 2004 Jul; 15(1):31.
Application:WB, Recombinant protein.
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Interaction of human DNA polymerase eta with monoubiquitinated PCNA: a possible mechanism for the polymerase switch in response to DNA damage.
Kannouche PL, Wing J, Lehmann AR.
Molecular Cell 2004 May; 14(4):491.
Application:WB, Human, MRC5 cells.
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Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA.
Haracska L, Unk I, Johnson RE, Phillips BB, Hurwitz J, Prakash L, Prakash S.
Molecular and Cellular Biology 2002 Feb; 22(3):784.
Application:Added, Recombinant protein.
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A defined human system that supports bidirectional mismatch-provoked excision.
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