PCNA monoclonal antibody, clone PC10
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Hela cells with PCNA monoclonal antibody, clone PC10 (Cat # MAB12946) followed by HRP-conjugated goat anti-mouse secondary antibody and visualized by chemiluminescence detection system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with PCNA monoclonal antibody, clone PC10 (Cat # MAB12946) at 10 ug/mL working concentration.
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Specifications
Product Description
Mouse monoclonal antibody raised against recombinant human PCNA.
Immunogen
Recombinant protein corresponding to human PCNA.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG2a
Recommend Usage
Flow Cytometry
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.09% sodium azide).
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Hela cells with PCNA monoclonal antibody, clone PC10 (Cat # MAB12946) followed by HRP-conjugated goat anti-mouse secondary antibody and visualized by chemiluminescence detection system.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with PCNA monoclonal antibody, clone PC10 (Cat # MAB12946) at 10 ug/mL working concentration.Immunoprecipitation
Flow Cytometry
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Gene Info — PCNA
Entrez GeneID
5111Protein Accession#
P12004Gene Name
PCNA
Gene Alias
MGC8367
Gene Description
proliferating cell nuclear antigen
Omim ID
176740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
Other Designations
DNA polymerase delta auxiliary protein|OTTHUMP00000030189|OTTHUMP00000030190|cyclin
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Interactomes
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Pathways
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Diseases
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