PCNA polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
PCNA polycolnal antibody (Cat # PAB27564) (0.05ug/ml) staining of Jurkat lysate. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot
PCNA polycolnal antibody (Cat # PAB27564) (0.05ug/ml) staining of Lane 1: NIH3T3, Lane 2: mouse testis and Lane 3: rat testis (35ug protein in RIPA). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of PCNA.
Immunogen
A synthetic peptide corresponding to amino acids 111-122 at internal region of human PCNA.
Sequence
EAPNQEKVSDYE
Host
Goat
Theoretical MW (kDa)
30
Reactivity
Chicken, Dog, Human, Mouse, Pig, Rat
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Western Blot (0.05-0.2ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PCNA polycolnal antibody (Cat # PAB27564) (0.05ug/ml) staining of Jurkat lysate. Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot
PCNA polycolnal antibody (Cat # PAB27564) (0.05ug/ml) staining of Lane 1: NIH3T3, Lane 2: mouse testis and Lane 3: rat testis (35ug protein in RIPA). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — PCNA
Entrez GeneID
5111Protein Accession#
NP_002583.1Gene Name
PCNA
Gene Alias
MGC8367
Gene Description
proliferating cell nuclear antigen
Omim ID
176740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
Other Designations
DNA polymerase delta auxiliary protein|OTTHUMP00000030189|OTTHUMP00000030190|cyclin
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Proteomic changes associated with maternal dietary low ω6:ω3 ratio in piglets supplemented with seaweed part II: Ileum proteomes.
Thi Xuan Nguyen, Alessandro Agazzi, Suzanne McGill, Stefan Weidt, Quang Hanh Han, Andrea Gelemanović, Mark McLaughlin, Giovanni Savoini, Peter David Eckersall, Richard Burchmore.
Journal of Proteomics 2023 Sep; 270:104739.
Application：WB-Ce, Piglet, Piglet ileum.
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Proteomic changes associated with maternal dietary low ω6:ω3 ratio in piglets supplemented with seaweed part II: Ileum proteomes.
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