PCNA monoclonal antibody, clone 1157CT1.1.3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell line lysates Lane 2: mouse L929 cell line lysates Lane 3: rat C6 cell line lysates reacted with PCNA monoclonal antibody (Cat # MAB12342) at 1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human lymph section reacted with PCNA monoclonal antibody (Cat # MAB12342) at 1:25 dilution.
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Specifications
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human PCNA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 236-261 at C-terminus region of human PCNA.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (1:25)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell line lysates Lane 2: mouse L929 cell line lysates Lane 3: rat C6 cell line lysates reacted with PCNA monoclonal antibody (Cat # MAB12342) at 1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human lymph section reacted with PCNA monoclonal antibody (Cat # MAB12342) at 1:25 dilution. -
Gene Info — PCNA
Entrez GeneID
5111Gene Name
PCNA
Gene Alias
MGC8367
Gene Description
proliferating cell nuclear antigen
Omim ID
176740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
Other Designations
DNA polymerase delta auxiliary protein|OTTHUMP00000030189|OTTHUMP00000030190|cyclin
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Interactomes
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Pathways
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Diseases
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