PCNA monoclonal antibody, clone PCNA/694
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of HepG2 cell lysate with PCNA monoclonal antibody, clone PCNA/694 (Cat # MAB13394).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with PCNA monoclonal antibody, clone PCNA/694 (Cat # MAB13394).
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Specifications
Product Description
Mouse monoclonal antibody raised against full length recombinant human PCNA.
Immunogen
Recombinant protein corresponding to full length human PCNA.
Host
Mouse
Theoretical MW (kDa)
36
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western Blot analysis of HepG2 cell lysate with PCNA monoclonal antibody, clone PCNA/694 (Cat # MAB13394).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with PCNA monoclonal antibody, clone PCNA/694 (Cat # MAB13394).Immunofluorescence
Flow Cytometry
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Gene Info — PCNA
Entrez GeneID
5111Protein Accession#
P12004Gene Name
PCNA
Gene Alias
MGC8367
Gene Description
proliferating cell nuclear antigen
Omim ID
176740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
Other Designations
DNA polymerase delta auxiliary protein|OTTHUMP00000030189|OTTHUMP00000030190|cyclin
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Interactomes
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Pathways
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Diseases
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