ACTB monoclonal antibody, clone BF-1
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Specifications
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human ACTB.
Immunogen
A synthetic peptide corresponding to human ACTB.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:3000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1)HeLa cell lysate; (2)Human fetal kidney lysate; (3) NIH/3T3 cell lysate; (4) PC-12 cell lysate with ACTB monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded (1) Human heart; (2) Mouse testis; (3) Human bladder cancer; (4) Mouse heart with ACTB monoclonal antibody.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of Hela cells with ACTB monoclonal antibody. -
Gene Info — ACTB
Entrez GeneID
60Protein Accession#
P60709Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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