ACTB/ACTG1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ACTB/ACTG1 polyclonal antibody (Cat # PAB27013) in extracts from Raw264.7 cells at 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of ACTB/ACTG1 polyclonal antibody (Cat # PAB27013) in paraffin-embedded human skeletal muscle tissue.
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACTB/ACTG1.
Immunogen
A synthetic peptide corresponding to human ACTB/ACTG1.
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Human, Mouse, Rat
Specificity
ACTB/ACTG1 polyclonal antibody detects endogenous levels of ACTB/ACTG1 protein.
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ACTB/ACTG1 polyclonal antibody (Cat # PAB27013) in extracts from Raw264.7 cells at 1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of ACTB/ACTG1 polyclonal antibody (Cat # PAB27013) in paraffin-embedded human skeletal muscle tissue.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACTB
Entrez GeneID
60Protein Accession#
P60709 (Gene ID : 60);P63261 (Gene ID : 71)Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Gene Info — ACTG1
Entrez GeneID
71Protein Accession#
P60709 (Gene ID : 60);P63261 (Gene ID : 71)Gene Name
ACTG1
Gene Alias
ACT, ACTG, DFNA20, DFNA26
Gene Description
actin, gamma 1
Gene Ontology
HyperlinkGene Summary
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. [provided by RefSeq
Other Designations
actin, cytoplasmic 2|actin, gamma 1 propeptide|cytoskeletal gamma-actin
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Interactomes
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Pathways
- Adherens junction
- Adherens junction
- Arrhythmogenic right ventricular cardiomyopathy (ARVC)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC)
- Focal adhesion
- Focal adhesion
- Hypertrophic cardiomyopathy (HCM)
- Hypertrophic cardiomyopathy (HCM)
- Leukocyte transendothelial migration
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Diseases
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