
beta-actin monoclonal antibody, clone RM112

* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against of human beta-actin.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to N-terminus region of human beta-actin.
Sequence
N/A
Specificity
This antibody reacts to beta-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:200)
Immunohistochemistry (1:200)
Immunoprecipitation (1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of A431 cells using beta-actin monoclonal antibody, clone RM112 (Cat # MAB12828) at 1:1000 dilution.Immunohistochemistry
Immunohistochemistry
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue using beta-actin monoclonal antibody, clone RM112 (Cat# MAB12828) at a 1:1000 dilution.Immunocytochemistry
Immunocytochemistry staining of A431 cells with beta-actin monoclonal antibody, clone RM112 (Cat # MAB12828) (Red) at 1:200 dilution. Nuclear DNA was stained with DAPI (Blue).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
-
Gene Info — ACTB
Entrez GeneID
60Protein Accession#
P60709Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
-
Interactomes
-
Pathways
-
Diseases
-
Publication Reference
-
GM130-silencing may aggravate blood-brain barrier damage and affect microglia polarization by down-regulating PD-L1 expression after experimental intracerebral hemorrhage.
Xiqian Chen, Yijun Ren, Pinghui Xie, Qiang Lei, Wei Lu.
Mol Biol Rep. 2024 Aug 19;51(1):919. doi: 10.1007/s11033-024-09859-x. 2024 Aug; 51(1):919.
Application:WB, Rat, Brain tissue.
-
Momordica anti-HIV protein MAP30 abrogates the Epstein-Barr virus nuclear antigen 1 dependent functions in host cells.
Wei-Hang Huang, Wen-Min Su, Chung-Wei Wang, Yue-Hao Fang, Yuan-Wei Jian, Hao-Jen Hsu, Chih-Wen Peng.
Heliyon 2023 Oct; 9(11):e21486.
Application:WB, Human, AKATA, BJAB, LCL cells.
-
Dual peptide-dendrimer conjugate inhibits acetylation of transforming growth factor β-induced protein and improves survival in sepsis.
Wonhwa Lee, Eun Ji Park, Oh Kwang Kwon, Hyelim Kim, Youngbum Yoo, Shin-Woo Kim, Young-Kyo Seo, In-San Kim, Dong Hee Na, Jong-Sup Bae.
Biomaterials 2020 Jul; 246:120000.
Application:WB-Ce, Human, HUVECs.
-
Autosomal Dominant PTH Gene Signal Sequence Mutation in a Family with Familial Isolated Hypoparathyroidism.
Cinque L, Sparaneo A, Penta L, Mencarelli A, Rogaia D, Esposito S, Fabrizio FP, Baorda F, Verrotti A, Falorni A, Stangoni G, Hendy GN, Guarnieri V, Prontera P.
The Journal of Clinical Endocrinology and Metabolism 2017 Nov; 102(11):3961.
Application:WB-Tr, Human, HEK 293 cells.
-
GM130-silencing may aggravate blood-brain barrier damage and affect microglia polarization by down-regulating PD-L1 expression after experimental intracerebral hemorrhage.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com