ACTB monoclonal antibody, clone 137CT26.1.1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 whole cell lysates Lane 2: C2C12 whole cell lysates Lane 3: C6 whole cell lysates Lane 4: Hela whole cell lysates Lane 5: MCF-7 whole cell lysates reacted with ACTB monoclonal antibody (Cat # MAB12291) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human skeletal muscle section reacted with ACTB monoclonal antibody (Cat # MAB12291) at 1:25 dilution.
Immunofluorescence
Immunofluorescent staining of Hela cell reacted with ACTB monoclonal antibody (Cat # MAB12291) at 1:10-1:50 dilution.
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Specifications
Product Description
Mouse monoclonal antibody raised against human ACTB.
Immunogen
Recombinant protein corresponding to human ACTB.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Immunohistochemistry (1:25)
Immunofluorescence (1:10-1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 whole cell lysates Lane 2: C2C12 whole cell lysates Lane 3: C6 whole cell lysates Lane 4: Hela whole cell lysates Lane 5: MCF-7 whole cell lysates reacted with ACTB monoclonal antibody (Cat # MAB12291) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human skeletal muscle section reacted with ACTB monoclonal antibody (Cat # MAB12291) at 1:25 dilution.Immunofluorescence
Immunofluorescent staining of Hela cell reacted with ACTB monoclonal antibody (Cat # MAB12291) at 1:10-1:50 dilution. -
Gene Info — ACTB
Entrez GeneID
60Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Increased acute blood flow induced by the aqueous extract of Euterpe oleracea Mart. fruit pulp in rats in vivo is not related to the direct activation of endothelial cells.
Victória Caroline Bottino Pontes, Juliana Pereira Tavares de Melo Tavares, Tatiana Rosado Rosenstock, Domingos Sávio Rodrigues, Marcelo Icimoto Yudi, Jaqueline Pereira Moura Soares, Suzana Cosa Ribeiro, Rafael Sutti, Luce Maria Brandão Torres, Fabiana Henriques Machado de Melo, Maria Thereza Gamberini.
Journal of Ethnopharmacology 2021 Feb; 271:113885.
Application：WB-Ce, Rat, Rat endothelial cells.
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Increased acute blood flow induced by the aqueous extract of Euterpe oleracea Mart. fruit pulp in rats in vivo is not related to the direct activation of endothelial cells.
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