EGFR (phospho Y1197) polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of EGFR.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y1197 of human EGFR.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
Reactivity occurs against human EGFR pY1197 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human EGFR is minimal by ELISA.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000-1:20000)
Western Blot (1:250-1:1500)
Immunohistochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using EGFR (phospho Y1197) polyclonal antibody (Cat # PAB10009) shows detection of a band at ~170 KDa corresponding to human EGFR (arrowhead).
Staining is not seen in unstimulated A-431 cells (Lane 1), but is seen when A-431 cells are stimulated with EGF (50 ng/mL for 15 min) (lane 2). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 250.
Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of EGFR (phospho Y1197) polyclonal antibody (Cat # PAB10009) was used at 5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows faintly to moderately positive staining of placental trophoblasts at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communi-cation, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EGFR
Entrez GeneID
1956Protein Accession#
P00533;NP_005219Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Role of epidermal growth factor receptor and STAT-3 activation in autonomous proliferation of SUM-102PT human breast cancer cells.
Sartor CI, Dziubinski ML, Yu CL, Jove R, Ethier SP.
Cancer Research 1997 Mar; 57(5):978.
Application:WB-Ce, Human, SUM-1O2PT cells.
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Expression of ras oncogene p21 protein in normal and neoplastic ovarian tissues: correlation with histopathologic features and receptors for estrogen, progesterone, and epidermal growth factor.
Scambia G, Catozzi L, Panici PB, Ferrandina G, Coronetta F, Barozzi R, Baiocchi G, Uccelli L, Piffanelli A, Mancuso S.
Am J Obstet Gynecol 1993 Jan; 168(1 Pt 1):71.
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Occurrence of epidermal growth factor receptors in benign and malignant ovarian tumors and normal ovarian tissues: an immunohistochemical study.
Henzen-Logmans SC, van der Burg ME, Foekens JA, Berns PM, Brussee R, Fieret JH, Klijn JG, Chadha S, Rodenburg CJ.
Journal of Cancer Research and Clinical Oncology 1992 Apr; 118(4):303.
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Role of epidermal growth factor receptor and STAT-3 activation in autonomous proliferation of SUM-102PT human breast cancer cells.
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