EGFR (phospho Y1068) recombinant monoclonal antibody, clone 1B8
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: A549 whole cell lysate (treated with Pervanadate), Lane 2: A549 whole cell lysate (not treated), Lane 3: HepG2 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 4: HepG2 whole cell lysate (not treated) with EGFR (phospho Y1068) recombinant monoclonal antibody, clone 1B8 (Cat # RAB04266).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EGFR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Y1068 of human EGFR.
Theoretical MW (kDa)
Calculated MW: 175 k
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: A549 whole cell lysate (treated with Pervanadate), Lane 2: A549 whole cell lysate (not treated), Lane 3: HepG2 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 4: HepG2 whole cell lysate (not treated) with EGFR (phospho Y1068) recombinant monoclonal antibody, clone 1B8 (Cat # RAB04266).Enzyme-linked Immunoabsorbent Assay
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Gene Info — EGFR
Entrez GeneID
1956Protein Accession#
P00533Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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