Products

Western Blot

Immunohistochemistry

Immunocytochemistry

Immunofluorescence

Immunofluorescent staining of NIH3T3 cells with EGFR recombinant monoclonal antibody, clone S3 12D (Cat # RAB03428).
Immunofluorescence analysis of unpermearaformaldehyde NIH3T3 cells fixed on Shi-fix™ coverslips stained with the chimeric rsion of RAB03428 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with unimmunised r followed by staining with Alexa Fluor® 488 secondary antibody.

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometric analysis of mouse splenocytes with EGFR recombinant monoclonal antibody, clone S3 12D (Cat # RAB03428).
Mouse splenocytes were stained with unimmunized r antibody (black line) or the rmeric version of RAB03428 (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing the bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/mL and cells analyzed using a FACSCanto flow-cytometer.

Entrez GeneID

1956

Gene Name

EGFR

Gene Alias

ERBB, ERBB1, HER1, PIG61, mENA

Gene Description

epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

Omim ID

131550 211980

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq

Other Designations

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor