EGFR purified MaxPab rabbit polyclonal antibody (D01P)
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against a full-length human EGFR protein.
Immunogen
EGFR (NP_958440.1, 1 a.a. ~ 405 a.a) full-length human protein.
Sequence
MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGLS
Host
Rabbit
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (89)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
EGFR MaxPab rabbit polyclonal antibody. Western Blot analysis of EGFR expression in A-431.Western Blot (Transfected lysate)
Western Blot analysis of EGFR expression in transfected 293T cell line (H00001956-T01) by EGFR MaxPab polyclonal antibody.
Lane 1: EGFR transfected lysate(44.70 KDa).
Lane 2: Non-transfected lysate.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between EGFR and CTNNA1. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-CTNNA1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of purified MaxPab antibody to EGFR on MCF-7 cell. [antibody concentration 10 ug/ml]Immunofluorescence
Immunofluorescence of purified MaxPab antibody to EGFR on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — EGFR
Entrez GeneID
1956GeneBank Accession#
NM_201283Protein Accession#
NP_958440.1Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactomes
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Pathways
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Diseases
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