CREB1 (phospho S133) recombinant monoclonal antibody, clone 3C11
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate and Lane 2: 293 whole cell lysate with CREB1 (phospho S133) recombinant monoclonal antibody, clone 3C11 (Cat # RAB04290).
Immunohistochemistry
Immunohistochemical staining of human lung cancer with CREB1 (phospho S133) recombinant monoclonal antibody, clone 3C11 (Cat # RAB04290) (diluated at 1:100)
Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with CREB1 (phospho S133) recombinant monoclonal antibody, clone 3C11 (Cat # RAB04290) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of Hela cells with CREB1 (phospho S133) recombinant monoclonal antibody, clone 3C11 (Cat # RAB04290) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CREB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S133 of human CREB1.
Theoretical MW (kDa)
Calculated MW: 46 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate and Lane 2: 293 whole cell lysate with CREB1 (phospho S133) recombinant monoclonal antibody, clone 3C11 (Cat # RAB04290).Immunohistochemistry
Immunohistochemical staining of human lung cancer with CREB1 (phospho S133) recombinant monoclonal antibody, clone 3C11 (Cat # RAB04290) (diluated at 1:100)Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with CREB1 (phospho S133) recombinant monoclonal antibody, clone 3C11 (Cat # RAB04290) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of Hela cells with CREB1 (phospho S133) recombinant monoclonal antibody, clone 3C11 (Cat # RAB04290) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CREB1
Entrez GeneID
1385Protein Accession#
P16220Gene Name
CREB1
Gene Alias
CREB, MGC9284
Gene Description
cAMP responsive element binding protein 1
Omim ID
123810Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
active transcription factor CREB|cAMP-response element-binding protein-1|transactivator protein
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Interactome
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Pathway
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Disease
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