ATF2 (phospho T71) recombinant monoclonal antibody, clone 4F2
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More Files
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Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate (treated with Calyculin A 50 nM/60 mins), Lane 2: 293 whole cell lysate (not treated), Lane 3: A549 whole cell lysate (treated with EGF 100 ng/mL/20 mins), Lane 4: A549 whole cell lysate (not treated), Lane 5: HepG2 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 6: HepG2 whole cell lysate (not treated) with ATF2 (phospho T71) recombinant monoclonal antibody, clone 4F2 (Cat # RAB04248).
Immunofluorescence
Immunofluorescent staining of A549 cells with ATF2 (phospho T71) recombinant monoclonal antibody, clone 4F2 (Cat # RAB04248) (diluated at 1:63). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ATF2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding T71 of human ATF2.
Theoretical MW (kDa)
Calculated MW: 70 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate (treated with Calyculin A 50 nM/60 mins), Lane 2: 293 whole cell lysate (not treated), Lane 3: A549 whole cell lysate (treated with EGF 100 ng/mL/20 mins), Lane 4: A549 whole cell lysate (not treated), Lane 5: HepG2 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 6: HepG2 whole cell lysate (not treated) with ATF2 (phospho T71) recombinant monoclonal antibody, clone 4F2 (Cat # RAB04248).Immunofluorescence
Immunofluorescent staining of A549 cells with ATF2 (phospho T71) recombinant monoclonal antibody, clone 4F2 (Cat # RAB04248) (diluated at 1:63). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATF2
Entrez GeneID
1386Protein Accession#
P15336Gene Name
ATF2
Gene Alias
CRE-BP1, CREB2, HB16, MGC111558, TREB7
Gene Description
activating transcription factor 2
Omim ID
123811Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000163262|activating transcription factor 2 splice variant ATF2-var2|cAMP responsive element binding protein 2, formerly
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