ATF2 recombinant monoclonal antibody, clone 3D12
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: K562 whole cell lysate and Lane 2: 293T whole cell lysate with ATF2 recombinant monoclonal antibody, clone 3D12 (Cat # RAB04352).
Immunohistochemistry
Immunohistochemical staining of human prostate tissue with ATF2 recombinant monoclonal antibody, clone 3D12 (Cat # RAB04352) (diluated at 1:115.5).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ATF2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ATF2.
Theoretical MW (kDa)
Calculated MW: 55, 3
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: K562 whole cell lysate and Lane 2: 293T whole cell lysate with ATF2 recombinant monoclonal antibody, clone 3D12 (Cat # RAB04352).Immunohistochemistry
Immunohistochemical staining of human prostate tissue with ATF2 recombinant monoclonal antibody, clone 3D12 (Cat # RAB04352) (diluated at 1:115.5).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATF2
Entrez GeneID
1386Protein Accession#
P15336Gene Name
ATF2
Gene Alias
CRE-BP1, CREB2, HB16, MGC111558, TREB7
Gene Description
activating transcription factor 2
Omim ID
123811Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000163262|activating transcription factor 2 splice variant ATF2-var2|cAMP responsive element binding protein 2, formerly
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Interactome
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Pathway
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Disease
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