PRKCA recombinant monoclonal antibody, clone R09-7C7
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More Files
- More Functions
Western Blot
Western blot analysis of Phospho-PKC alpha (Thr638) in K562, C6 lysates using human Phospho-PKC alpha (Thr638) recombinant monoclonal antibody, clone R09-7C7 (Cat # RAB01670).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with Phospho-PKC alpha (Thr638) recombinant monoclonal antibody, clone R09-7C7 (Cat # RAB01670). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic phosphopeptide corresponding to residues surrounding Thr638 of human PKC alpha.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr638 of human PKC alpha
Theoretical MW (kDa)
Calculated MW: 77 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1,000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Phospho-PKC alpha (Thr638) in K562, C6 lysates using human Phospho-PKC alpha (Thr638) recombinant monoclonal antibody, clone R09-7C7 (Cat # RAB01670).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with Phospho-PKC alpha (Thr638) recombinant monoclonal antibody, clone R09-7C7 (Cat # RAB01670). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunoprecipitation
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Gene Info — PRKCA
Entrez GeneID
5578Protein Accession#
P17252Gene Name
PRKCA
Gene Alias
AAG6, MGC129900, MGC129901, PKC-alpha, PKCA, PRKACA
Gene Description
protein kinase C, alpha
Omim ID
176960Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq
Other Designations
aging-associated gene 6|protein kinase C alpha type
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Interactome
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Pathway
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Disease
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