PRKCA (phospho T638) recombinant monoclonal antibody, clone 3A5
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More Files
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Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate (not treated), Lane 2: Hela whole cell lysate (treated with EGF 100ng/ml/20mins), Lane 3: A549 whole cell lysate (not treated) and Lane 4: A549 whole cell lysate (treated with Calyculin A 100nM/60 mins) with PRKCA (phospho T638) recombinant monoclonal antibody, clone 3A5 (Cat # RAB04282).
Immunofluorescence
Immunofluorescent staining of HepG2 cells with PRKCA (phospho T638) recombinant monoclonal antibody, clone 3A5 (Cat # RAB04282) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PRKCA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding T638 of human PRKCA.
Theoretical MW (kDa)
Calculated MW: 80 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate (not treated), Lane 2: Hela whole cell lysate (treated with EGF 100ng/ml/20mins), Lane 3: A549 whole cell lysate (not treated) and Lane 4: A549 whole cell lysate (treated with Calyculin A 100nM/60 mins) with PRKCA (phospho T638) recombinant monoclonal antibody, clone 3A5 (Cat # RAB04282).Immunofluorescence
Immunofluorescent staining of HepG2 cells with PRKCA (phospho T638) recombinant monoclonal antibody, clone 3A5 (Cat # RAB04282) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCA
Entrez GeneID
5578Protein Accession#
P17252Gene Name
PRKCA
Gene Alias
AAG6, MGC129900, MGC129901, PKC-alpha, PKCA, PRKACA
Gene Description
protein kinase C, alpha
Omim ID
176960Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq
Other Designations
aging-associated gene 6|protein kinase C alpha type
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Interactome
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Disease
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