ADORA2A polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ADORA2A.
Immunogen
A synthetic peptide corresponding to human ADORA2A.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to ADORA2A.
Form
Liquid
Recommend Usage
Western Blot (1:500~1:1000)
Immunohistochemistry (1:50~1:100)
Immunofluorescence (1:500~1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using ADORA2A polyclonal antibody (Cat # PAB18061).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ADORA2A polyclonal antibody (Cat # PAB18061).
Peptide "+" means "with peptide blocking".Immunohistochemistry
Immunofluorescence
Immunofluorescence analysis of LoVo cells, using ADORA2A polyclonal antibody (Cat # PAB18061).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — ADORA2A
Entrez GeneID
135Protein Accession#
P29274Gene Name
ADORA2A
Gene Alias
ADORA2, RDC8, hA2aR
Gene Description
adenosine A2a receptor
Omim ID
102776Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. [provided by RefSeq
Other Designations
adenosine A2 receptor|adenosine receptor subtype A2a
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Interactomes
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Pathways
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Diseases
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Publication Reference
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A genome annotation-driven approach to cloning the human ORFeome.
Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I.
Genome Biol 2004 Sep; 5(10):R84.
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Characterization and chromosomal localization of the human A2a adenosine receptor gene: ADORA2A.
Le F, Townsend-Nicholson A, Baker E, Sutherland GR, Schofield PR.
Biochemical and Biophysical Research Communications 1996 Jun; 223(2):461.
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Molecular characterization of a human brain adenosine A2 receptor.
Furlong TJ, Pierce KD, Selbie LA, Shine J.
Brain Research. Molecular Brain Research 1992 Sep; 15(1-2):62.
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A genome annotation-driven approach to cloning the human ORFeome.
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