ADORA2A monoclonal antibody, clone 6A6G11
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ADORA2A-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with ADORA2A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ADORA2A monoclonal antibody, clone 6A6G11.
Flow Cytometry
Flow cytometric analysis of Hela cells with ADORA2A monoclonal antibody (green) and negative control (red).
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Specifications
Product Description
Mouse monoclonal antibody raised against recombinant human ADORA2A.
Immunogen
Recombinant protein corresponding to amino acid 274-412 of human ADORA2A from E. coli.
Host
Mouse
Theoretical MW (kDa)
44.7
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunocytochemistry (1:100-1:500)
Flow Cytometry (1:200-1:400)
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of (1) HEK293 cells, (2) ADORA2A-hIgGFc transfected HEK293 cell lysate.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ADORA2A monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ADORA2A monoclonal antibody, clone 6A6G11.Flow Cytometry
Flow cytometric analysis of Hela cells with ADORA2A monoclonal antibody (green) and negative control (red). -
Gene Info — ADORA2A
Entrez GeneID
135Gene Name
ADORA2A
Gene Alias
ADORA2, RDC8, hA2aR
Gene Description
adenosine A2a receptor
Omim ID
102776Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. [provided by RefSeq
Other Designations
adenosine A2 receptor|adenosine receptor subtype A2a
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Interactomes
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Pathways
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Diseases
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