MMP1 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human placenta stained with MMP1 polyclonal antibody (Cat # PAB12708) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MMP1.
Immunogen
A synthetic peptide corresponding to C-terminus of human MMP1.
Host
Rabbit
Theoretical MW (kDa)
72
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~72 KDa of MMP1 (Interstitial collagenase/ gelatinase A).
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human placenta stained with MMP1 polyclonal antibody (Cat # PAB12708) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MMP1
Entrez GeneID
4312Gene Name
MMP1
Gene Alias
CLG, CLGN
Gene Description
matrix metallopeptidase 1 (interstitial collagenase)
Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq
Other Designations
fibroblast collagenase|interstitial collagenase|matrix metalloprotease 1|matrix metalloproteinase 1|matrix metalloproteinase 1 (interstitial collagenase)
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss.
Thanh-Tam Tran, Gyuseok Lee, Yun Hyun Huh, Ki-Ho Chung, Sun Young Lee, Ka Hyon Park, Seung Hee Kwon, Min-Suk Kook, Jang-Soo Chun, Jeong-Tae Koh, Je-Hwang Ryu.
Experimental & Molecular Medicine 2023 Dec; 55(12):2553.
Application：WB, Human, Gingival fibroblasts.
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Matrix metalloproteinase expression is altered in the small and large intestine following fractionated radiation in vivo.
Stansborough RL, Al-Dasooqi N, Bateman EH, Bowen JM, Keefe DMK, Logan RM, Yeoh ASJ, Yeoh EEK, Stringer AM, Gibson RJ.
Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in 2018 May; [Epub].
Application：IHC, Rat, Jejunal, Colonic tissues.
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The Expression of Matrix Metalloproteinases in Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL)-expressing Cancer of Apocrine Origin.
Kambayashi Y, Fujimura T, Furudate S, Lyu C, Hidaka T, Kakizaki A, Sato Y, Tanita K, Aiba S.
Anticancer Research 2018 Jan; 38(1):113.
Application：IHC-P, Human, Human primary cutaneous apocrine carcinoma.
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Wnt5a induces catabolic signaling and matrix metalloproteinase production in human articular chondrocytes.
Huang G, Chubinskaya S, Liao W, Loeser RF.
Osteoarthritis and Cartilage 2017 Jun; 25(9):1505.
Application：WB, Human, Human chondrocytes.
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Osteoarthritic changes in vervet monkey knees correlate with meniscus degradation and increased matrix metalloproteinase and cytokine secretion.
Stone AV, Vanderman KS, Willey JS, Longc DL, Register TC, Shively CA, Stehle Jr. JR, Loeser RF, Ferguson CM.
Osteoarthritis and Cartilage 2015 Oct; 23(10):1780.
Application：WB, Monkey, Monkey meniscal explant cultures.
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Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis pathogenesis.
Stone AV, Loeser RF, Vanderman KS, Long DL, Clark SC, Ferguson CM.
Osteoarthritis and Cartilage 2014 Feb; 22(2):264.
Application：WB, Human, Cartilage, Meniscus cells.
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BTG2 inhibits the proliferation, invasion, and apoptosis of MDA-MB-231 triple-negative breast cancer cells.
Zhang YJ, Wei L, Liu M, Li J, Zheng YQ, Gao Y, Li XR.
Tumor Biology 2013 Jun; 34(3):1605.
Application：WB, Human, MDA-MB-231 cells.
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Effect of ARHI on lung cancer cell proliferation, apoptosis and invasion in vitro.
Wu X, Liang L, Dong L, Yu Z, Fu X.
Molecular Biology Reports 2012 Dec; 40(3):2671.
Application：WB-Ce, Human, SK-MES-1 cells.
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Urine proteomic analysis differentiates cholangiocarcinoma from primary sclerosing cholangitis and other benign biliary disorders.
Metzger J, Negm AA, Plentz RR, Weismuller TJ, Wedemeyer J, Karlsen TH, Dakna M, Mullen W, Mischak H, Manns MP, Lankisch TO.
Gut 2012 May; 62(1):122.
Application：IHC-P, Human, Liver tissues from patients with cholangiocarcinoma (CC) and primary sclerosing cholangitis (PSC).
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Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss.
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