IFNA1 (Human) Recombinant Protein
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More Files
- More Functions
Surface Plasmon Resonance
Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human IFN alpha 1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IFN alpha 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human IFNA1 (P01562, Cys24-Glu189) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Cys24-Glu189
Host
Human
Theoretical MW (kDa)
46.14
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IFN alpha 1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IFN alpha 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
SPR
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). -
Gene Info — IFNA1
Entrez GeneID
3439Protein Accession#
P01562Gene Name
IFNA1
Gene Alias
IFL, IFN, IFN-ALPHA, IFNA13, IFNA@, MGC138207, MGC138505, MGC138507
Gene Description
interferon, alpha 1
Omim ID
147660Gene Ontology
HyperlinkGene Summary
Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon (IFN-gamma; MIM 147570) is produced by mitogen- or antigen-stimulated T lymphocytes.[supplied by OMIM
Other Designations
IFN-alpha 1b|OTTHUMP00000045110|interferon alpha 1b
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Interactome
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Pathway
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Disease
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