IFNA1 (Human) Recombinant Protein
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Specification
Product Description
Human IFNA1 recombinant protein with polyhistidine tag at the N-terminus expressed in Escherichia coli.
Sequence
CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE with polyhistidine tag at the N-terminus.
Host
Escherichia coli
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 95% as determined by SDS-PAGE.
Endotoxin Level
< 0.1 EU/ ug of protein by the LAL method.
Activity
ED50 < 1.12 ug/mL, measured by the ability to inhibit IL-8 secretion in human PBMCs in the presence of LPS.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of IFNA1 (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 8.0. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year.
Avoid repeated freeze/thaw cycles.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — IFNA1
Entrez GeneID
3439Gene Name
IFNA1
Gene Alias
IFL, IFN, IFN-ALPHA, IFNA13, IFNA@, MGC138207, MGC138505, MGC138507
Gene Description
interferon, alpha 1
Omim ID
147660Gene Ontology
HyperlinkGene Summary
Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon (IFN-gamma; MIM 147570) is produced by mitogen- or antigen-stimulated T lymphocytes.[supplied by OMIM
Other Designations
IFN-alpha 1b|OTTHUMP00000045110|interferon alpha 1b
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