EGFR recombinant monoclonal antibody, clone Matuzumab
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunofluorescence
Immunofluorescence analysis of were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/mL and cells analyzed on a FACSCanto flow-cytometer.
Enzyme-linked Immunoabsorbent Assay
ELISA Plate coated with human EGFR-Fc at a concentration of 5 ug/mL. A 3-fold serial dilution from 100 to 0.1 ng/mL was performed using. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.
Flow Cytometry
Flow-cytometry were stainedwere stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/mL and cells analyzed on a FACSCanto flow-cytometer.
-
Specification
Product Description
Mouse recombinant monoclonal antibody raised against human EGFR.
Antibody Species
Mouse
Immunogen
Original antibody is raised against by immunizing BALB/c mice intraperitoneally with A431 cells in phosphate buffered saline (PBS). Later on the humanzied version of the antibody was created by grafting CDRs of the murine antibody onto human constant regions.
Reactivity
Human
Specificity
This antibody binds the extracellular domain III of the human EGFR and does not cross react with murine EGFR. It was also reported that residues S460/G461 in EGFR domain III are essential components of the epitope. This antibody binds at a site distinct from the EGF binding site, preventing conformational re-arrangement required for dimerization, a process essential for high-affinitiy ligand binding (Schmiedel, 2008). This antibody can only recognize the active form of EGFR. The antibody binds to both glyco- and aglyco receptor forms. EGFR is a transmembrane mitogenic glycoprotein with a cytoplasmically facing tyrosine kinase site which is activated by binding of the epidermal growth factor in the external domain. It also binds transforming growth factor alpha (TGFa). EGFR a member of the epidermal growth factor family and is closely related to receptor tyrosine kinases. This receptor tyrosine kinase binds ligands of the EGF family and activates signallying cascades to convert extrac
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG1, Kappa
Recommend Usage
ELISA
Flow cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
Applications
Immunofluorescence
Immunofluorescence analysis of were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/mL and cells analyzed on a FACSCanto flow-cytometer.Enzyme-linked Immunoabsorbent Assay
ELISA Plate coated with human EGFR-Fc at a concentration of 5 ug/mL. A 3-fold serial dilution from 100 to 0.1 ng/mL was performed using. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.Flow Cytometry
Flow-cytometry were stainedwere stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (blue line) at a concentration of 10 ug/mL for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/mL and cells analyzed on a FACSCanto flow-cytometer. -
Gene Info — EGFR
Entrez GeneID
1956Protein Accession#
P00533Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com