CD40LG (Human) Recombinant Protein
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Lane 1: non-reducing conditions
Lane 2: reducing conditions
Result of activity analysis
Result of activity analysis
Serial dilutions of human CD40LG, starting at 2000 ng/mL, were added to human PBMCs. After 48 hours, cell supernatant was collected and human IL-8 was measured via ELISA and the linear portion of the curve was us used to calculate the ED50.
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Specifications
Product Description
Human CD40LG (P29965) recombinant protein expressed in Escherichia coli.
Sequence
MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
Host
Escherichia coli
Theoretical MW (kDa)
16.3
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Endotoxin Level
< 0.1 EU/ug
Activity
The activity is determined by the dose-dependent production of IL-8 from human PBMCs. The expected ED50 for this effect is 8-12 ng/mL.
Quality Control Testing
1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditionsStorage Buffer
Lyophilized from 10 mM Na2PO4, pH 7.5
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CD40LG
Entrez GeneID
959Protein Accession#
P29965Gene Name
CD40LG
Gene Alias
CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Gene Description
CD40 ligand
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq
Other Designations
CD40 antigen ligand|OTTHUMP00000024130|T-B cell-activating molecule|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5
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Interactomes
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Pathways
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Diseases
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