CD40LG Trimer (Human) Recombinant Protein (Biotin)
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.70 ug/mL determined by ELISA.
Tris-Bis PAGE
Biotinylated Human CD40LG Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human CD40LG Trimer is greater than 95% as determined by SEC-HPLC.
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Specifications
Product Description
Human CD40LG Trimer (P29965, Met113-Leu261) partial recombinant protein with His-Flag tag at the N-Terminus expressed in HEK293 cells.
Sequence
Met113-Leu261
Host
Human
Theoretical MW (kDa)
52.4
Reactivity
Human
Form
Lyophilized
Conjugation
Biotin
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by Tris-Bis PAGE
> 95% by HPLCEndotoxin Level
< 0.1 EU per 1 ug as determined by the LAL method.
Activity
The EC50 was 0.70 ug/mL, measured by ELISA at 2 ug/mL.
Recommend Usage
Biological Activity
ELISA
Tris-Bis PAGE
SEC-HPLC
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. (8% trehalose).
Storage Instruction
Store at -20°C for 12 months.
After reconstitution, store at 4°C for 2-7 days, or store at -80°C for 3-6 months.
Aliquot to avoid repeated freezing and thawing.Note
Please use within one month after protein reconstitution.
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.70 ug/mL determined by ELISA.Functional Study
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Gene Info — CD40LG
Entrez GeneID
959Protein Accession#
P29965Gene Name
CD40LG
Gene Alias
CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Gene Description
CD40 ligand
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq
Other Designations
CD40 antigen ligand|OTTHUMP00000024130|T-B cell-activating molecule|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5
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Interactomes
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Pathways
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Diseases
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