CD40LG (Human) Recombinant Protein
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More Files
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Inhibition Assay
Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.
ELISA
Immobilized Human CD40, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 9.0ng/ml determined by ELISA (QC Test).
SEC-HPLC
The purity of Human CD40 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD40 Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specifications
Product Description
Human CD40LG (P29965, Met113-Leu261) partial recombinant protein with monomeric hFc tag at N-terminus expressed in HEK293 cells.
Sequence
Met113-Leu261
Host
Human
Theoretical MW (kDa)
74.90000000000001
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Recommend Usage
Biological Activity
ELISA
SEC-HPLC
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from filtered solution in PBS, pH 7.4 (5% trehalose).
Storage Instruction
After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Inhibition Assay
Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.Functional Study
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Gene Info — CD40LG
Entrez GeneID
959Protein Accession#
P29965Gene Name
CD40LG
Gene Alias
CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Gene Description
CD40 ligand
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq
Other Designations
CD40 antigen ligand|OTTHUMP00000024130|T-B cell-activating molecule|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5
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Interactomes
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Pathways
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Diseases
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