EGFR polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EGFR.
Immunogen
A synthetic peptide corresponding to human EGFR.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to EGFR.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HUVEC cells, treated with Serum (20 %, 30 mins), using EGFR polyclonal antibody (Cat # PAB18169).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR polyclonal antibody (Cat # PAB18169).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HepG2 cells, using EGFR polyclonal antibody (Cat # PAB18169).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — EGFR
Entrez GeneID
1956Protein Accession#
P00533Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Tumor endothelial cells express epidermal growth factor receptor (EGFR) but not ErbB3 and are responsive to EGF and to EGFR kinase inhibitors.
Amin DN, Hida K, Bielenberg DR, Klagsbrun M.
Cancer Research 2006 Feb; 66(4):2173.
Application:Flow Cyt, IF, IHC-Fr, WB, Human, Mouse, A375, Human breast carcinoma, melanoma, MDA-MB-231 tumor xenografts, Mouse skin.
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Metaplastic breast carcinomas are negative for Her-2 but frequently express EGFR (Her-1): potential relevance to adjuvant treatment with EGFR tyrosine kinase inhibitors?
Leibl S, Moinfar F.
Journal of Clinical Pathology 2005 Jul; 58(7):700.
Application:IHC-P, Human, Human bresat cancer.
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Tumor endothelial cells express epidermal growth factor receptor (EGFR) but not ErbB3 and are responsive to EGF and to EGFR kinase inhibitors.
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