ACTB monoclonal antibody, clone AC-15
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against synthetic peptide of ACTB.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus amoino acids (Ac-DDDIAALVIDNGSGK) of human ACTB.
Host
Mouse
Theoretical MW (kDa)
42
Reactivity
Human, Mouse, Rat
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Western Blot (0.25-0.5 &mciro;g/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 1.2% sodium acetate (2 mg BSA, 0.01 mg sodium azide)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 1 mL of 1.2% sodium acetate or neutral PBS and concentration will be 100 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of rat skeletal muscle with ACTB monoclonal antibody, clone AC-15 (Cat # MAB8232). -
Gene Info — ACTB
Entrez GeneID
60Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Interactomes
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Pathways
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Diseases
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Publication Reference
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AC133/CD133/Prominin-1.
Shmelkov SV, St Clair R, Lyden D, Rafii S.
The International Journal of Biochemistry & Cell Biology 2005 Apr; 37(4):715.
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Rat prominin, like its mouse and human orthologues, is a pentaspan membrane glycoprotein.
Corbeil D, Fargeas CA, Huttner WB.
Biochem Biophys Res Commun 2001 Jul; 285(4):939.
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The human AC133 hematopoietic stem cell antigen is also expressed in epithelial cells and targeted to plasma membrane protrusions.
Corbeil D, Roper K, Hellwig A, Tavian M, Miraglia S, Watt SM, Simmons PJ, Peault B, Buck DW, Huttner WB.
The Journal of Biological Chemistry 2000 Feb; 275(8):5512.
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AC133/CD133/Prominin-1.
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