IL18 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
IL18 polyclonal antibody (Cat # PAB14391) (0.1 ug/mL) staining of HeLa (1), (2ug/ml) A431 (2) and US0S (3) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence
IL18 polyclonal antibody (Cat # PAB14391) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunofluorescence
IL18 polyclonal antibody (Cat # PAB14391) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry
IL18 polyclonal antibody (Cat # PAB14391) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of IL18.
Immunogen
A synthetic peptide corresponding to human IL18.
Sequence
C-NPPDNIKDTKSDI
Host
Goat
Theoretical MW (kDa)
22.3
Reactivity
Human
Specificity
Approx 23 KDa band observed in human placenta lysates and in lysates of cell line HeLa (calculated MW of 22.3 KDa according to NP_001553.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Immunofluorescence (10 ug/mL)
Flow Cytometry (10 ug/mL)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
IL18 polyclonal antibody (Cat # PAB14391) (0.1 ug/mL) staining of HeLa (1), (2ug/ml) A431 (2) and US0S (3) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunofluorescence
IL18 polyclonal antibody (Cat # PAB14391) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Immunofluorescence
IL18 polyclonal antibody (Cat # PAB14391) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi and cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
IL18 polyclonal antibody (Cat # PAB14391) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — IL18
Entrez GeneID
3606Protein Accession#
NP_001553.1Gene Name
IL18
Gene Alias
IGIF, IL-18, IL-1g, IL1F4, MGC12320
Gene Description
interleukin 18 (interferon-gamma-inducing factor)
Omim ID
600953Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. [provided by RefSeq
Other Designations
IL-1 gamma|interferon-gamma-inducing factor|interleukin 18|interleukin-1 gamma|interleukin-18
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Interactome
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Pathway
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Disease
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Publication Reference
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Regulatory polymorphisms in the interleukin-18 promoter are associated with hepatitis C virus clearance.
An P, Thio CL, Kirk GD, Donfield S, Goedert JJ, Winkler CA.
The Journal of Infectious Diseases 2008 Oct; 198(8):1159.
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Regulatory polymorphisms in the interleukin-18 promoter are associated with hepatitis C virus clearance.
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