Abnova has extensive experience in generating high quality antisera to a wide variety of antigens (peptides, phospho-peptides, proteins, and DNAs). Using a low dose immunization technique in combination with a proprietary adjuvant, we are able to generate a high-titer, high-affinity antibody response against the immunogen in mice, rats, and rabbits.

custom_service_pab.pdf

Polyclonal Antibody Development Workflow
  • Evaluation of Standard Antibody Production Service
  • Pre-Production Discussion: Custom Specification, Flowchart, Cost
  • Purchase Order
  • Production Specification Approval
  • Production Specification Template
  • Polyclonal Antibody Development and Progress Report
  • Polysera Delivery

 

 

Mouse & Rat

Service Items

Time (weeks)

Antigen Supplied by Customer

      Antigen Supplied by Abnova
 Peptide Phospho-peptide  Protein DNA
Peptide Design & Synthesis

Customer-supplied antigen

4 ~ 5

  		 V V V              

7~20-mer (Purity > 80%)  

       V V V        

Single site phosphorylation
(Ser, Tyr, Thr)

           V        
Conjugation
To carrier protein KLH/BSA/OVA
(A cysteine is automatically added to the C-terminus or N-terminus of the peptide)		 1       V   V        
Protein Preparation
DNA sourcing    2 ~ 3                         V V        
Cloning and sequencing
Protein expression
DNA Preparation
DNA sourcing 2 ~ 3                 V V
Cloning and sequencing
Immunization
5~8 injections per animal
(3 mice or 3rats)		 8 ~ 12  V V    V   V V V V V V V
Sera collection
Report
ELISA data * 1 V V V V V V V V V V
WB data (mammalian overexpression lysate)                 V V
Purification **
By protein A purification
Final deliverable: > 0.2 mg purified antibody for mice or > 5 mg purified antibody for rats		 2   V   V     V   V  
By affinity purification
(for rats only)
Final deliverable: > 0.5 mg purified antibody		     V   V V   V   V
Total Lead time (weeks) 10~12 12~14 12~14 17~20 17~20 17~20 19~20 19~20 12~16 12~16
Total Price (USD/EUR) Quote Quote Quote Quote Quote Quote Quote Quote Quote Quote

 

Rabbit

Service Items

Time (weeks)

Antigen Supplied by Customer

      Antigen Supplied by Abnova
 Peptide Phospho-peptide  Protein DNA
Peptide Design & Synthesis

Customer-supplied antigen

4 ~ 5

  		 V V V              

7~20-mer (Purity > 80%)  

       V V V        

Single site phosphorylation
(Ser, Tyr, Thr)

           V        
Conjugation
To carrier protein KLH/BSA/OVA
(A cysteine is automatically added to the C-terminus or N-terminus of the peptide)		 1       V  V V        
Protein Preparation
DNA sourcing    2 ~ 3                         V V        
Cloning and sequencing
Protein expression
DNA Preparation
DNA sourcing 2 ~ 3                 V V
Cloning and sequencing
Immunization
5~8 injections per rabbit
(2 rabbits) 		10 ~ 12  V V    V   V V V V V V V
Sera collection
Report
ELISA data * 1 V V V V V V V V V V
WB data (mammalian overexpression lysate)                 V V
Purification **
By protein A purification
Final deliverable: 20-50 mg purified antibody		 2   V   V     V   V  
By affinity purification
Final deliverable: 1-5 mg purified antibody		     V   V V   V   V
Total Lead time (weeks) 11~14 13~16 13~16 17~20 17~20 17~20 19~20 19~20 12~16 12~16
Total Price (USD/EUR) Quote Quote Quote Quote Quote Quote Quote Quote Quote Quote

Note:
* Polyclonal antibody sera will be QC utilizing ELISA format for reactivity against the immunogen.
** This is the average yield. Actual yield will vary for each Pab.

 

The above list price is only used for direct orders made to Abnova.


 
 

For any inquiry, please contact : OEM@abnova.com

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