Mouse, Rat & Rabbit Polyclonal Ab (All)

Abnova has extensive experience in generating high-quality antisera to a wide variety of antigens (peptides, phospho-peptides, proteins, and DNAs). Using a low-dose immunization technique in combination with a proprietary adjuvant, we are able to generate a high-titer, high-affinity antibody response against the immunogen in mice, rats, and rabbits.

custom_service_pab.pdf

Polyclonal Antibody Development Workflow

Evaluation of Standard Antibody Production Service
Pre-Production Discussion: Custom Specification, Flowchart, Cost
Purchase Order
Production Specification Approval
Production Specification Template
Polyclonal Antibody Development and Progress Report
Polysera Delivery

Mouse & Rat

Service Items	Time (weeks)	Antigen Supplied by Customer			Antigen Supplied by Abnova
Service Items	Time (weeks)	Antigen Supplied by Customer			Peptide		Phospho-peptide	Protein		DNA
Peptide Design & Synthesis
Customer-supplied antigen	4 ~ 5	V	V	V
7~20-mer (Purity > 80%)					V	V	V
Single site phosphorylation (Ser, Tyr, Thr)							V
Conjugation
To carrier protein KLH/BSA/OVA (A cysteine is automatically added to the C-terminus or N-terminus of the peptide)	1				V		V
Protein Preparation
DNA sourcing	2 ~ 3							V	V
Cloning and sequencing
Protein expression
DNA Preparation
DNA sourcing	2 ~ 3									V	V
Cloning and sequencing	2 ~ 3									V	V
Immunization
5~8 injections per animal (3 mice or 3rats)	8 ~ 12	V	V	V	V	V	V	V	V	V	V
Sera collection	8 ~ 12	V	V	V	V	V	V	V	V	V	V
Report
ELISA data *	1	V	V	V	V	V	V	V	V	V	V
WB data (mammalian overexpression lysate)	1									V	V
Purification **
By protein A purification Final deliverable: > 0.2 mg purified antibody for mice or > 5 mg purified antibody for rats	2		V		V			V		V
By affinity purification (for rats only) Final deliverable: > 0.5 mg purified antibody	2			V		V	V		V		V
Total Lead time (weeks)		10~12	12~14	12~14	17~20	17~20	17~20	19~20	19~20	12~16	12~16
Total Price (USD/EUR)		Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote

Rabbit

Service Items	Time (weeks)	Antigen Supplied by Customer			Antigen Supplied by Abnova
Service Items	Time (weeks)	Antigen Supplied by Customer			Peptide		Phospho-peptide	Protein		DNA
Peptide Design & Synthesis
Customer-supplied antigen	4 ~ 5	V	V	V
7~20-mer (Purity > 80%)					V	V	V
Single site phosphorylation (Ser, Tyr, Thr)							V
Conjugation
To carrier protein KLH/BSA/OVA (A cysteine is automatically added to the C-terminus or N-terminus of the peptide)	1				V	V	V
Protein Preparation
DNA sourcing	2 ~ 3							V	V
Cloning and sequencing
Protein expression
DNA Preparation
DNA sourcing	2 ~ 3									V	V
Cloning and sequencing	2 ~ 3									V	V
Immunization
5~8 injections per rabbit (2 rabbits)	10 ~ 12	V	V	V	V	V	V	V	V	V	V
Sera collection	10 ~ 12	V	V	V	V	V	V	V	V	V	V
Report
ELISA data *	1	V	V	V	V	V	V	V	V	V	V
WB data (mammalian overexpression lysate)	1									V	V
Purification **
By protein A purification Final deliverable: 20-50 mg purified antibody	2		V		V			V		V
By affinity purification Final deliverable: 1-5 mg purified antibody	2			V		V	V		V		V
Total Lead time (weeks)		11~14	13~16	13~16	17~20	17~20	17~20	19~20	19~20	12~16	12~16
Total Price (USD/EUR)		Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote	Quote

Note:
* Polyclonal antibody sera will be QC utilizing ELISA format for reactivity against the immunogen.
** This is the average yield. Actual yield will vary for each Pab.

The above list price is only used for direct orders made to Abnova.

For any inquiry, please contact : OEM@abnova.com

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